Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/35848

TitleUsing an in-vitro biofilm model to assess the virulence potential of Bacterial Vaginosis or non-Bacterial Vaginosis Gardnerella vaginalis isolates
Author(s)Castro, Joana
Alves, Patrícia Maria
Sousa, Cármen Sofia Vieira
Cereija, Tatiana
França, Ângela Maria Oliveira Sousa
Jefferson, Kimberly K.
Cerca, Nuno
KeywordsReverse transcription polymerase chain reaction
Biofilms
Infection
Urogenital reproductive disorders
Issue dateJun-2015
PublisherNature Publishing Group
JournalScientific Reports
CitationCastro, Joana; Alves, P.; Sousa, Cármen; Cereija, T.B.; França, A.; Jefferson, K.K.; Cerca, N., Using an in-vitro biofilm model to assess the virulence potential of Bacterial Vaginosis or non-Bacterial Vaginosis Gardnerella vaginalis isolates. Scientific Reports, 5(11640), 2015
Abstract(s)Gardnerella vaginalis is the most common species found in bacterial vaginosis (BV). However, it is also present in a significant proportion of healthy women and G. vaginalis vaginal colonization does not always lead to BV. In an effort to better understand the differences between G. vaginalis isolated from women with a positive (BV) versus a negative (non-BV) diagnosis of BV, we compared the virulence potential of 7 BV and 7 non-BV G. vaginalis isolates and assessed the virulence factors related to biofilm formation, namely: initial adhesion and cytotoxic effect, biofilm accumulation, susceptibility to antibiotics, and transcript levels of the known vaginolysin, and sialidase genes. Furthermore, we also determined the ability of G. vaginalis to displace lactobacilli previously adhered to HeLa cells. Our results showed that non-BV strains were less virulent than BV strains, as suggested by the lower cytotoxicity and initial adhesion to Hela cells. Significant differences in expression of known virulence genes were also detected, further suggesting a higher virulence potential of the BV associated G. vaginalis. Importantly, we demonstrated that BV associated G. vaginalis were able to displace pre-coated vaginal protective lactobacilli and we hypothesize this to be a trigger for BV development.
TypeArticle
URIhttp://hdl.handle.net/1822/35848
DOI10.1038/srep11640
ISSN2045-2322
e-ISSN2045-2322
Publisher versionhttp://www.nature.com/srep/2015/150626/srep11640/full/srep11640.html
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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