Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/35453

TítuloChondrosia reniformis marine-sponge collagen membranes for skin re-epithelialization
Autor(es)Prata, Margarida Barros
Silva, Joana M.
Marques, A. P.
Silva, Tiago H.
Reis, R. L.
Palavras-chaveChondrosia reniformis
Marine-sponge collagen
Skin re-epithelialization
DataJun-2014
EditoraJohn Wiley & Sons, Ltd.
CitaçãoPrata M. B., Moreira-Silva J., Marques A. P., Silva T. H., Reis R. L. Chondrosia reniformis marine-sponge collagen membranes for skin re-epithelialization, Journal of Tissue Engineering and Regenerative Medicine, 2014
Resumo(s)Chondrosia reniformis collagen has been identified as mainly of type IV. Being collagen IV the main component of the epidermal basal layer [1], C. reniformis represents a valuable source to be explored in the skin regeneration field. This work envisaged the production of C. reniformis collagen membranes for the selection of rapidly adherent epidermal cells, like the commercial collagen coatings, and for their subsequent culture. This approach would permit a single system for culturing and carrying basal epidermal cells aimed at re-epithelialize skin wounds. Materials and Methods The collagen of C. reniformis marine-sponge was extracted with 100mM Tris-HCl, 10mM EDTA, 8M urea and 100mM 2-mercaptoethanol. To define the best re-solubilization conditions, the obtained precipitate was dissolved in five different solutions: Solution A: 100mM Tris-HCl+8M Urea+10mM EDTA (pH 9.5); Solution B: 50mM Tris-HCl+1M NaCl (pH 7.4); Solution C: 100mM Tris-HCl (pH 7.4); Solution D: 0.5% H2O2 (v/v) (pH 11) and Solution E: 100mM Tris-HCl (pH 9.5). Solutions of 1% collagen were prepared and cross-linking was performed with HMDI, genipin and EDC/NHS at different concentrations. The membranes were obtained by solvent-casting and/or freeze-drying, and their stability was tested both in PBS and culture medium, for at least 7 days. Morphological characterization of the membranes was carried out by scanning electron microscopy (SEM). Cytotoxicity, based on metabolic activity (MTS assay) and cell proliferation (DNA quantification) analysis of the 100mM Tris-HCl (pH 9.5) and 8mM EDC/NHS cross-linked collagen membranes, was assessed with L929 cells. Results were analyzed by IBM SPSS Statistics Version 20 using one-way ANOVA and Kruskall-Wallis test. Significance was set for p
TipoconferenceAbstract
URIhttp://hdl.handle.net/1822/35453
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
18225-Prata Termis EU 2014.pdf122,77 kBAdobe PDFVer/Abrir

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu Currículo DeGóis