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|Title:||Orange IV stabilizes silk fibroin microemulsions|
|Author(s):||Ferreira, A. V.|
Abreu, Ana S.
Azóia, Nuno G.
Botelho, C. M.
Paulo, Artur Cavaco
|Publisher:||John Wiley and Sons|
|Journal:||Engineering in Life Sciences|
|Citation:||Ferreira, Ana V.; Volkov, V.; Abreu, A. S.; Azoia, Nuno G.; Botelho, C. M.; Paulo, Artur Cavaco, Orange IV stabilizes silk fibroin microemulsions. Engineering in Life Sciences, 15(4), 400-409, 2015|
|Abstract(s):||Silk fibroin (SF) is a natural biopolymer that has been extensively studied in various applications due to its impressive mechanical properties and biocompatibility. Recently, SF-based particles have been proposed as controlled drug delivery systems. A new and efficient method to prepare SF microemulsions (SF-MEs) was developed by oil-in-water emulsions using high-pressure homogenization to promote emulsification. During SF-ME production, the secondary structure of SF changed to a more stable conformation (from random coil to -sheets), thus allowing the formation of small and stable (140.7 ± 1.9 nm; polydispersity index, 0.25) SF microparticles (SF-MPs). The efficiency of SF-MP formation was 60%. Orange IV was used as a model compound for incorporation and release studies, although its incorporation into the SF-MEs significantly improved particle size and size distribution over at least 4 wk compared to traditional stabilizers (e.g., poloxamer 407, transcutol, Tween 80, and SDS). This should be a call of attention when using dyes as model compounds since they can influence particle properties and lead to misinterpretation of the results. Orange IV showed an incorporation efficiency of 91% and a controlled release over time. Stable SF-MP formulations, further enhanced by orange IV incorporation, provide an innovative method with potential application in pharmaceutical development due to its associated high biocompatibility and release profile.|
|Appears in Collections:||CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series|
IPC - Artigos em revistas científicas internacionais com arbitragem