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TitleCellulase recycling in biorefineriesis : is it possible?
Author(s)Gomes, Daniel Gonçalves
Rodrigues, Ana Cristina
Domingues, Lucília
Gama, F. M.
Enzyme recycling
Cellulase-substrate interactions
Lignocellulosic bioethanol
Cellulases cost
Issue dateMay-2015
JournalApplied Microbiology and Biotechnology
CitationGomes, D. G.; Rodrigues, Ana Cristina; Domingues, Lucília; Gama, F. M., Cellulase recycling in biorefineriesis it possible?. Applied Microbiology and Biotechnology, 99(10), 4131-4143, 2015
Abstract(s)On a near future, bio-based economy will assume a key role in our lives. Lignocellulosic materials (e.g., agroforestry residues, industrial/solid wastes) represent a cheaper and environmentally friendly option to fossil fuels. Indeed, following suitable processing, they can be metabolized by different microorganisms to produce a wide range of compounds currently obtained by chemical synthesis. However, due to the recalcitrant nature of these materials, they cannot be directly used by microorganisms, the conversion of polysaccharides into simpler sugars being thus required. This conversion, which is usually undertaken enzymatically, represents a significant part on the final cost of the process. This fact has driven intense efforts on the reduction of the enzyme cost following different strategies. Here, we describe the fundamentals of the enzyme recycling technology, more specifically, cellulase recycling. We focus on the main strategies available for the recovery of both the liquid- and solid-bound enzyme fractions and discuss the relevant operational parameters (e.g., composition, temperature, additives, and pH). Although the efforts from the industry and enzyme suppliers are primarily oriented toward the development of enzyme cocktails able to quickly and effectively process biomass, it seems clear by now that enzyme recycling is technically possible.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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