Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/35078
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Zago, Chaiene Evelin | por |
dc.contributor.author | Silva, Sónia Carina | por |
dc.contributor.author | Sanitá, Paula Volpato | por |
dc.contributor.author | Barbugli, Paula Aboud | por |
dc.contributor.author | Dias, Carla Maria Improta | por |
dc.contributor.author | Lordello, Virgínia Barreto | por |
dc.contributor.author | Vergani, Carlos Eduardo | por |
dc.date.accessioned | 2015-05-07T11:36:47Z | - |
dc.date.available | 2015-05-07T11:36:47Z | - |
dc.date.issued | 2015-04 | - |
dc.date.submitted | 2014-11 | - |
dc.identifier.citation | Zago, Chaiene Evelin; Silva, Sónia Carina; Sanitá, Paula Volpato; Barbugli, Paula Aboud; Dias, Carla Maria Improta; Lordello, Virgínia Barreto; Vergani, Carlos Eduardo, Dynamics of biofilm formation and the interaction between Candida albicans and Methicillin-susceptible (MSSA) and -Resistant Staphylococcus aureus (MRSA). PLoS One, 10(4), e0123206, 2015 | por |
dc.identifier.issn | 1932-6203 | por |
dc.identifier.uri | https://hdl.handle.net/1822/35078 | - |
dc.description.abstract | Polymicrobial biofilms are an understudied and a clinically relevant problem. This study evaluates the interaction between C. albicans, and methicillin- susceptible (MSSA) and resistant (MRSA) S. aureus growing in single- and dual-species biofilms. Single and dual species adhesion (90 min) and biofilms (12, 24, and 48 h) were evaluated by complementary methods: counting colony-forming units (CFU mL-1), XTT-reduction, and crystal violet staining (CV). The secretion of hydrolytic enzymes by the 48 h biofilms was also evaluated using fluorimetric kits. Scanning electron microscopy (SEM) was used to assess biofilm structure. The results from quantification assays were compared using two-way ANOVAs with Tukey post-hoc tests, while data from enzymatic activities were analyzed by one-way Welch-ANOVA followed by Games-Howell post hoc test ( = 0.05). C. albicans, MSSA and MRSA were able to adhere and to form biofilm in both single or mixed cultures. In general, all microorganisms in both growth conditions showed a gradual increase in the number of cells and metabolic activity over time, reaching peak values between 12 h and 48 h (<0.05). C. albicans single- and dual-biofilms had significantly higher total biomass values (<0.05) than single biofilms of bacteria. Except for single MRSA biofilms, all microorganisms in both growth conditions secreted proteinase and phospholipase-C. SEM images revealed extensive adherence of bacteria to hyphal elements of C. albicans. C. albicans, MSSA, and MRSA can co-exist in biofilms without antagonism and in an apparent synergistic effect, with bacteria cells preferentially associated to C. albicans hyphal forms. | por |
dc.description.sponsorship | CNPq (Council for Technical and Scientific Development) (Grant 400658/2012-7) | por |
dc.description.sponsorship | Fundação para a Ciência e Tecnologia (FCT), Portugal (SFRH/BPD/71076/2010) | por |
dc.description.sponsorship | CAPES(Coordination for the Improvement of Higher Level Personnel) | por |
dc.language.iso | eng | por |
dc.publisher | Public Library of Science | por |
dc.rights | openAccess | por |
dc.title | Dynamics of biofilm formation and the interaction between Candida albicans and methicillin-susceptible (MSSA) and -resistant Staphylococcus aureus (MRSA) | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0123206 | por |
dc.comments | CEB19982 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 15 | por |
oaire.citationIssue | 4 | por |
oaire.citationConferencePlace | United States | - |
oaire.citationTitle | PLoS ONE | por |
oaire.citationVolume | 10 | por |
dc.date.updated | 2015-05-06T10:45:43Z | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.doi | 10.1371/journal.pone.0123206 | por |
dc.identifier.pmid | 25875834 | por |
dc.subject.fos | Engenharia e Tecnologia::Engenharia Médica | por |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | PLoS ONE | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_19982_1.pdf | 2,42 MB | Adobe PDF | Ver/Abrir |