Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/35046

TitleAdhesion of adipose-derived mesenchymal stem cells to glycosaminoglycan surfaces with different protein patterns
Author(s)Costa, Diana Soares da
Márquez-Posadas, M. C.
Araújo, A. R.
Yang, Y.
Merino, S.
Groth, T.
Reis, R. L.
Pashkuleva, I.
Keywordsadipose-derived stem cells
CD44
Glycosaminoglycans
micropatterning
Issue dateApr-2015
PublisherAmerican Chemical Society
JournalACS Applied Materials and Interfaces
CitationSoares da Costa D., Márquez-Posadas M. C., Araújo A. R., Yang Y., Merino S., Groth T., Reis R. L., Pashkuleva I. Adhesion of adipose-derived mesenchymal stem cells to glycosaminoglycan surfaces with different protein patterns, ACS Applied Materials & Interfaces, Vol. 7, doi:10.1021/acsami.5b02479, 2015
Abstract(s)Proteins and glycosaminoglycans (GAGs) are main constituents of the extracellular matrix (ECM). They act in synergism and are equally critical for the development, growth, function or survival of an organism. In this work, we developed surfaces that display these two classes of biomacromolecules, namely GAGs and proteins, in spatially controlled fashion. The generated surfaces can be used as a minimalistic but straightforward model aiding the elucidation of cell-ECM interactions. GAGs (hyaluronic acid and heparin) were covalently bound to amino func-tionalized surfaces and albumin or fibronectin were patterned by micro-contact printing on top of them. We demonstrate that adipose-derived stem cells (ASCs) can adhere either on protein or GAG pattern as a function of the patterned molecules. ASCs found on the GAG pattern had different morphology and expressed different surface markers than the cells adherent on the protein pattern. ASCs morphology and spreading were also dependent on the size of the pattern. These results show that the developed supports can be also used for ASCs differentiation into different lineages.
TypeArticle
DescriptionUncorrected proof
URIhttp://hdl.handle.net/1822/35046
DOI10.1021/acsami.5b02479
ISSN1944-8244
e-ISSN1944-8252
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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