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TitleCompartmentalized bioencapsulated liquefied 3D macro-construct by perfusion-based layer-by-layer technique
Author(s)Praveen, S.
Correia, Clara R.
Costa, Rui R.
Mano, J. F.
Cell microenvironments
Encapsulation Technologies
Multilayer capsules
Tissue Constructs
Issue date2015
PublisherRoyal Society of Chemistry
JournalRSC Advances
CitationSher, P., Correia, C. R., Costa, R. R., & Mano, J. F. (2015). Compartmentalized bioencapsulated liquefied 3D macro-construct by perfusion-based layer-by-layer technique. Rsc Advances, 5(4), 2511-2516. doi: 10.1039/c4ra11674g
Abstract(s)Self-supporting, millimeter length 3D constructs consisting of individualized liquefied compartments, were produced using cell encapsulated hydrogel beads as building blocks. A perfusion-based layer-by-layer approach was used that allowed bioencapsulated beads to assemble, pattern, hold and attach to produce non-liquefied 3D constructs with controlled shape, displaying the binding feature of a continuous nanometric multilayer coating. No binders or crosslinking strategies were used. The internal microenvironment of this 3D construct was modified from solid to liquefied state by chelation. MTS and liveâ dead assays showed enhanced L929 cell viability with liquefied 3D constructs, compared to non-liquefied ones. The proposed technique opens new prospects to create complex 3D polyelectrolyte based constructs for tissue engineering applications.
Publisher version!divAbstract
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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