Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/34934

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dc.contributor.authorMonteiro, Nelsonpor
dc.contributor.authorMartins, Margaridapor
dc.contributor.authorMartins, Albinopor
dc.contributor.authorFonseca, Nuno A.por
dc.contributor.authorMoreira, João N.por
dc.contributor.authorReis, R. L.por
dc.contributor.authorNeves, N. M.por
dc.date.accessioned2015-04-22T10:15:56Z-
dc.date.available2015-04-22T10:15:56Z-
dc.date.issued2015-
dc.identifier.issn1742-7061por
dc.identifier.urihttp://hdl.handle.net/1822/34934-
dc.description.abstractChitsan (Ch) nanofiber mesh (NFM) is a material with natural characteristics favoring its use in human wound dressing. The present work proposes a gentamicin-loaded liposome immobilized at the surface of Ch NFMs to promote its antibacterial activity. To achieve this purpose, Ch NFMs were functionalized with thiol groups, and gentamicin-loaded liposomes were covalently immobilized by the reaction of the SH groups with maleimide. The maximum concentration of SH groups (55.52 ± 11.19 nmol cm2) was obtained at pH 7. A fluorescent dye was covalently bound to the SH groups present at the surface of electrospun Ch NFMs. Their spatial distribution was uniform throughout the NFMs when analyzed by fluorescence microscopy. Gentamicin was successfully encapsulated into the liposomes with an efficiency of 17%. Gentamicin-loaded liposomes were uniformly distributed at the surface of the Ch NFMs and the drug release kinetic showed a sustained release of gentamicin during 16 h, achieving a steady state at 24 h. The in vitro susceptibility tests confirmed that the gentamicin released from the liposomes immobilized at the surface of electrospun Ch NFM has bactericidal activity against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The results show that the developed system has promising performance for wound dressing applications, avoiding infections caused by these common pathogens.por
dc.description.sponsorshipThe authors thank the Portuguese Foundation for Science and Technology for the PhD grant of N.S. Monteiro (SFRH/BD/62465/2009), the Post-Doc grants of A. Martins (SFRH/BPD/70669/2010) and M. Martins (SFRH/BPD/73663/2010). This work was supported by OsteoGraphy (PTDC/EME-MFE/2008) and MaxBone (PTDC/SAU-ENB/115179/2009) projects. It was also partly supported by the POLARIS (FP7-REGPOT-2012-20131) and RL3 - TECT - NORTE-01-0124-FEDER-000020, co-financed by North Portugal Regional Operational Programme (ON.2 - O Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF), and by the research grant from the Portuguese Society of Nephrology to Dr. Anabela Rodrigues.por
dc.language.isoengpor
dc.publisherElsevier B.V.por
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F62465%2F2009/PT-
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F70669%2F2010/PT-
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F73663%2F2010/PT-
dc.relationPTDC/EME-MFE/2008-
dc.relationinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/115179/PT-
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/316331/EU-
dc.rightsrestrictedAccesspor
dc.subjectChitosanpor
dc.subjectLiposomespor
dc.subjectGentamicinpor
dc.subjectWound dressingpor
dc.subjectDrug deliverypor
dc.titleAntibacterial activity of chitosan nanofiber meshes with liposomes immobilized releasing gentamicinpor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.journals.elsevier.com/acta-biomaterialia/por
dc.commentsCEB19876por
sdum.publicationstatuspublishedpor
oaire.citationStartPage196por
oaire.citationEndPage205por
oaire.citationConferencePlaceNetherlands-
oaire.citationTitleActa Biomaterialiapor
oaire.citationVolume18por
dc.date.updated2015-04-21T22:02:50Z-
dc.identifier.doi10.1016/j.actbio.2015.02.018por
dc.identifier.pmid25749293por
dc.subject.wosScience & Technologypor
sdum.journalActa Biomaterialiapor
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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