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|Title:||Enzymatic synthesis of poly(catechin)-antibiotic conjugates : an antimicrobial approach for indwelling catheters|
Abreu, Ana S.
Matamá, Maria Teresa
Ribeiro, Artur J.
Gomes, A. C.
Silva, Carla Manuela Pereira Marinho da
Paulo, Artur Cavaco
|Journal:||Applied Microbiology and Biotechnology|
|Abstract(s):||Biofilm formation in urinary indwelling catheters is one of the most critical issues that patients face. Catheters were coated with poly(catechin)-antibiotic conjugates with enhanced antimicrobial properties. Catechin was conjugated with two antibiotics, namely trimethoprim (TMP) and sulfamethoxazole (SMZ) via activation with N,N′-disuccinimidyl carbonate (DSC) and subsequent coupling to molecules containing α-amine moieties. Silicone and polyurethane catheters were functionalized in situ through laccase oxidation of catechin-antibiotic conjugates. Four antimicrobial coatings were produced, namely with poly(catechin), poly(catechin)- TMP, poly(catechin)-SMZ and poly(catechin)-TMP-SMZ. The bacterial adhesion reduction was tested on the functionalized devices using gram-negative and gram-positive strains. The most significant reduction in adhesion was observed with poly(catechin)-TMP (gram-negative −85 % and grampositive −87 %) and with poly(catechin)-TMP-SMZ (gramnegative −85 % and gram-positive −91 %). The cytotoxicity to mammalian cells was tested by indirect contact for 5 days and revealed that all the tested coatings supported more than 90 % of viable cells. A promising approach for the increase of the indwelling catheter lifespan was developed aiming to reduce catheter-associated chronic infections.|
|Appears in Collections:||3B’s - Artigos em revistas/Papers in scientific journals|
CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series
IPC - Artigos em revistas científicas internacionais com arbitragem
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