Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/34640

TitleMyoconductive and osteoinductive free-standing polysaccharide membranes
Author(s)Caridade, S. G.
Monge, C.
Almodóvar, J.
Guillot, R.
Lavaud, J.
Josserand, V.
Coll, J. L.
Mano, J. F.
Picart, Catherine
KeywordsBiomaterials
free-standing membranes
layer-by-layer
Polysaccharides
Tissue engineering
Osteoinduction
Issue dateJan-2015
PublisherElsevier
JournalActa Biomaterialia
CitationCaridade, S. G., Monge, C., Almodóvar, J., Guillot, R., Lavaud, J., Josserand, V., . . . Picart, C. (2015). Myoconductive and osteoinductive free-standing polysaccharide membranes. Acta Biomaterialia, 15, 139-149. doi: 10.1016/j.actbio.2014.12.027
Abstract(s)Free-standing (FS) membranes have increasing applications in the biomedical field as drug delivery systems for wound healing and tissue engineering. Here, we studied the potential of free-standing membranes made by the layer-by-layer assembly of chitosan and alginate to be used as a simple biomimetic system of the periosteum. The design of a periosteum-like membrane implies the elaboration of a thick membrane suitable for both muscle and bone formation. Our aim was to produce well-defined ∼50 μm thick polysaccharide membranes that could be easily manipulated, were mechanically resistant, and would enable both myogenesis and osteogenesis in vitro and in vivo. The membranes were chemically crosslinked to improve their mechanical properties. Crosslinking chemistry was followed via Fourier transform infrared spectroscopy and the mechanical properties of the membranes were assessed using dynamic mechanical analysis. The loading and release of the potent osteoinductive growth factor bone morphogenetic protein 2 (BMP-2) inside and outside of the FS membrane was followed by fluorescence spectroscopy in a physiological buffer over 1 month. The myogenic and osteogenic potentials of the membranes in vitro were assessed using BMP-2-responsive skeletal myoblasts. Finally, their osteoinductive properties in vivo were studied in a preliminary experiment using a mouse ectopic model. Our results showed that the more crosslinked FS membranes enabled a more efficient myoblast differentiation in myotubes. In addition, we showed that a tunable amount of BMP-2 can be loaded into and subsequently released from the membranes, depending on the crosslinking degree and the initial BMP-2 concentration in solution. Only the more crosslinked membranes were found to be osteoinductive in vivo. These polysaccharide-based membranes have strong potential as a periosteum-mimetic scaffold for bone tissue regeneration.
TypeArticle
URIhttp://hdl.handle.net/1822/34640
DOI10.1016/j.actbio.2014.12.027
ISSN1742-7061
Publisher versionhttp://www.sciencedirect.com/science/article/pii/S174270611400600X
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
18297-free-standing Acta Biomaterialia.pdf2,84 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID