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TitleBiosurfactant production by Bacillus subtilis using corn steep liquor as culture medium
Author(s)Gudiña, Eduardo J.
Fernandes, Elisabete Clara Resende
Rodrigues, A. I.
Teixeira, J. A.
Rodrigues, L. R.
KeywordsBacillus subtilis
Corn steep liquor
Microbial enhanced oil recovery
Issue dateFeb-2015
PublisherFrontiers Media
JournalFrontiers in microbiology
CitationGudiña, Eduardo J.; Fernandes, Elisabete C.; Rodrigues, Ana I.; Teixeira, J. A.; Rodrigues, L. R., Biosurfactant production by Bacillus subtilis using corn steep liquor as culture medium. Frontiers in Microbiology, 6(59), 2015
Abstract(s)In this work, biosurfactant production by Bacillus subtilis #573 was evaluated using corn steep liquor (CSL) as culture medium. The best results were obtained in a culture medium consisting of 10% (v/v) of CSL, with a biosurfactant production of about 1.3 g/l. To the best of our knowledge, this is the first report describing biosurfactant production by B. subtilis using CSL as culture medium. Subsequently, the effect of different metals (iron, manganese, and magnesium) on biosurfactant production was evaluated using the medium CSL 10%. It was found that for all the metals tested, the biosurfactant production was increased (up to 4.1, 4.4, and 3.5 g/l for iron, manganese, and magnesium, respectively). When the culture medium was supplemented with the optimum concentration of the three metals simultaneously, the biosurfactant production was increased up to 4.8 g/l. Furthermore, the biosurfactant exhibited a good performance in oil recovery assays when compared with chemical surfactants, which suggests its possible application in microbial enhanced oil recovery or bioremediation.
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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