Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/33628

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dc.contributor.authorSoares, João Brunopor
dc.contributor.authorNunes, Pedro Pimentelpor
dc.contributor.authorAfonso, Luíspor
dc.contributor.authorRolanda, Carlapor
dc.contributor.authorLopes, Paulapor
dc.contributor.authorRoncon-Albuquerque, R.por
dc.contributor.authorGonçalves, Nádiapor
dc.contributor.authorCarvalho, Inês Boalpor
dc.contributor.authorPardal, Fernandopor
dc.contributor.authorLopes, Susanapor
dc.contributor.authorMacedo,Guilhermepor
dc.contributor.authorHenrique, Ruipor
dc.contributor.authorDias, Rui Moreirapor
dc.contributor.authorGonçalves, Raquelpor
dc.contributor.authorRibeiro, Mário Dinispor
dc.contributor.authorMoreira, Adelino F. Leitepor
dc.date.accessioned2015-02-05T15:57:41Z-
dc.date.available2015-02-05T15:57:41Z-
dc.date.issued2012-
dc.identifier.issn1753-4259por
dc.identifier.urihttp://hdl.handle.net/1822/33628-
dc.descriptionPublicado em "Innate Immunity". ISSN 1753-4259, vol. 18, n.º 5 (2012), p. 700-708por
dc.description.abstractWe evaluated expression of TLR2, TLR4 and proinflammatory genes [NF-?B, TNF-a, cyclooxygenase-2 (COX-2)] in liver samples of patients in different stages of liver disease. Fifteen patients with unexplained transaminases elevation (reference group), 22 with viral chronic hepatitis (hepatitis group), 14 with virus-induced severe fibrosis/cirrhosis (cirrhosis group) and 10 with hepatocarcinoma (hepatocarcinoma group) were consecutively included in the study. Quantification of TLR2, TLR4, NF-?B, TNF-a and COX-2 mRNA was done by real-time RT-PCR and TLR2 and TLR4 protein expression was evaluated by immunohistochemistry. Compared with reference, TLR2 and TLR4 mRNA was increased in hepatitis (TLR2: 2.66?±?0.69; TLR4: 3.11?±?0.79; P?<?0.05) and cirrhosis (TLR2: 2.14?±?0.5; TLR4: 1.74?±?0.27; P?<?0.05) and decreased in hepatocarcinoma (TLR2: 0.48?±?0.15; TLR4: 0.54?±?0.10; P?<?0.05). This associated with increased TNF-a and COX-2 mRNA in hepatitis (TNF-a: 3.24?±?0.79; COX-2: 2.47?±?0.36; P?<?0.05) and cirrhosis (TNF-a: 1.73?±?0.28; COX-2: 1.8?±?0.35, P?<?0.05), whereas NF-?B mRNA was increased in hepatitis (2.42?±?0.31; P?<?0.05) and unchanged in cirrhosis (1.34?±?0.17; P?=?0.3). Hepatocarcinoma presented increased COX-2 mRNA (1.63?±?0.15; P?<?0.05) and maintained (at decreased levels) mRNA of NF-?B (0.52?±?0.12) and TNF-a (0.52?±?0.12; P?<?0.05, all genes). Immunohistochemistry confirmed increased expression of TLR2 and TLR4 in hepatitis and cirrhosis and maintained expression in hepatocarcinoma. Upregulation of TLR2, TLR4 and their proinflammatory mediators is associated with virus-induced hepatic IFC sequence.por
dc.language.isoengpor
dc.publisherSAGE Publicationspor
dc.rightsopenAccesspor
dc.subjectChronic hepatitispor
dc.subjectCirrhosispor
dc.subjectHepatocarcinomapor
dc.subjectTLR2por
dc.subjectTLR4por
dc.titleIncreased hepatic expression of TLR2 and TLR4 in the hepatic inflammation-fibrosis-carcinoma sequencepor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://ini.sagepub.com/content/18/5/700.abstractpor
oaire.citationStartPage700por
oaire.citationEndPage708por
oaire.citationIssue5por
oaire.citationTitleInnate Immunitypor
oaire.citationVolume18por
dc.date.updated2015-01-29T16:58:56Z-
dc.identifier.doi10.1177/1753425912436762por
dc.identifier.pmid22330637por
dc.subject.wosScience & Technologypor
sdum.journalInnate Immunitypor
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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