Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/33628
Título: | Increased hepatic expression of TLR2 and TLR4 in the hepatic inflammation-fibrosis-carcinoma sequence |
Autor(es): | Soares, João Bruno Nunes, Pedro Pimentel Afonso, Luís Rolanda, Carla Lopes, Paula Roncon-Albuquerque, R. Gonçalves, Nádia Carvalho, Inês Boal Pardal, Fernando Lopes, Susana Macedo,Guilherme Henrique, Rui Dias, Rui Moreira Gonçalves, Raquel Ribeiro, Mário Dinis Moreira, Adelino F. Leite |
Palavras-chave: | Chronic hepatitis Cirrhosis Hepatocarcinoma TLR2 TLR4 |
Data: | 2012 |
Editora: | SAGE Publications |
Revista: | Innate Immunity |
Resumo(s): | We evaluated expression of TLR2, TLR4 and proinflammatory genes [NF-?B, TNF-a, cyclooxygenase-2 (COX-2)] in liver samples of patients in different stages of liver disease. Fifteen patients with unexplained transaminases elevation (reference group), 22 with viral chronic hepatitis (hepatitis group), 14 with virus-induced severe fibrosis/cirrhosis (cirrhosis group) and 10 with hepatocarcinoma (hepatocarcinoma group) were consecutively included in the study. Quantification of TLR2, TLR4, NF-?B, TNF-a and COX-2 mRNA was done by real-time RT-PCR and TLR2 and TLR4 protein expression was evaluated by immunohistochemistry. Compared with reference, TLR2 and TLR4 mRNA was increased in hepatitis (TLR2: 2.66?±?0.69; TLR4: 3.11?±?0.79; P?<?0.05) and cirrhosis (TLR2: 2.14?±?0.5; TLR4: 1.74?±?0.27; P?<?0.05) and decreased in hepatocarcinoma (TLR2: 0.48?±?0.15; TLR4: 0.54?±?0.10; P?<?0.05). This associated with increased TNF-a and COX-2 mRNA in hepatitis (TNF-a: 3.24?±?0.79; COX-2: 2.47?±?0.36; P?<?0.05) and cirrhosis (TNF-a: 1.73?±?0.28; COX-2: 1.8?±?0.35, P?<?0.05), whereas NF-?B mRNA was increased in hepatitis (2.42?±?0.31; P?<?0.05) and unchanged in cirrhosis (1.34?±?0.17; P?=?0.3). Hepatocarcinoma presented increased COX-2 mRNA (1.63?±?0.15; P?<?0.05) and maintained (at decreased levels) mRNA of NF-?B (0.52?±?0.12) and TNF-a (0.52?±?0.12; P?<?0.05, all genes). Immunohistochemistry confirmed increased expression of TLR2 and TLR4 in hepatitis and cirrhosis and maintained expression in hepatocarcinoma. Upregulation of TLR2, TLR4 and their proinflammatory mediators is associated with virus-induced hepatic IFC sequence. |
Tipo: | Artigo |
Descrição: | Publicado em "Innate Immunity". ISSN 1753-4259, vol. 18, n.º 5 (2012), p. 700-708 |
URI: | https://hdl.handle.net/1822/33628 |
DOI: | 10.1177/1753425912436762 |
ISSN: | 1753-4259 |
Versão da editora: | http://ini.sagepub.com/content/18/5/700.abstract |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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soares jb_innate immun 2012.pdf | 1,95 MB | Adobe PDF | Ver/Abrir |