Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/33608

TitleDevelopment of an electrochemical aptasensor for the detection of human osteopontin
Author(s)Meirinho, Sofia G.
Dias, Luís G.
Peres, António M.
Rodrigues, L. R.
Issue date2014
PublisherElsevier
JournalProcedia engineering
CitationMeirinho, Sofia G.; Dias, Luís G.; Peres, António M.; Rodrigues, L. R., Development of an electrochemical aptasensor for the detection of human osteopontin. Procedia Engineering, 87, 316-319, 2014
Abstract(s)Electrochemical aptasensors, an emerging technology, enables the detection of protein biomarkers, which may be indicative of tumour activity. Osteopontin is a protein present in body fluids, being a possible biomarker since its overexpression has been related with breast cancer progression. An \RNA\ aptamer, described in the literature, with affinity for human osteopontin, was synthetized, immobilized in a microelectrode gold surface and used for development electrochemical aptasensor for human \OPN\ detection in standard solutions. Cyclic voltammetry results showed that this aptasensor allowed detecting human osteopontin with a detection limit of 8 nM, showing a satisfactory selectivity towards the target in the presence of others proteins, except for thrombin.
TypeConference paper
URIhttp://hdl.handle.net/1822/33608
DOI10.1016/j.proeng.2014.11.671
ISSN1877-7058
e-ISSN1877-7058
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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