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|Title:||Development of an electrochemical aptasensor for the detection of human osteopontin|
|Author(s):||Meirinho, Sofia G.|
Dias, Luís G.
Peres, António M.
Rodrigues, L. R.
|Citation:||Meirinho, Sofia G.; Dias, Luís G.; Peres, António M.; Rodrigues, L. R., Development of an electrochemical aptasensor for the detection of human osteopontin. Procedia Engineering, 87, 316-319, 2014|
|Abstract(s):||Electrochemical aptasensors, an emerging technology, enables the detection of protein biomarkers, which may be indicative of tumour activity. Osteopontin is a protein present in body fluids, being a possible biomarker since its overexpression has been related with breast cancer progression. An \RNA\ aptamer, described in the literature, with affinity for human osteopontin, was synthetized, immobilized in a microelectrode gold surface and used for development electrochemical aptasensor for human \OPN\ detection in standard solutions. Cyclic voltammetry results showed that this aptasensor allowed detecting human osteopontin with a detection limit of 8 nM, showing a satisfactory selectivity towards the target in the presence of others proteins, except for thrombin.|
|Appears in Collections:||CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series|