Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/33607| Título: | Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery |
| Autor(es): | Santos, J. A. L. Belo, Isabel Mota, M. Cabral, J. M. S. |
| Palavras-chave: | Cell disruption Freeze/thawing Sonication Escherichia coli Cytochrome b5 |
| Data: | 1996 |
| Editora: | Kluwer |
| Revista: | Bioseparation |
| Citação: | Santos, J. A. L.; Belo, Isabel; Mota, M.; Cabral, J. M. S., Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery. Bioseparation, 6, 81-89, 1996 |
| Resumo(s): | The influence of sonication power, suspension volume and cell concentration on the kinetics of cytochrome b5 and intracellular protein release by sonication of Escherichia coli TB1 cells was studied. The influence of freezing and thawing of the cell suspension was also evaluated. Freezing and thawing increased the recovery yield of cytochrome b5. The sonication efficiency increased with the increase of sonication power and with the decrease of the suspension volume and cell concentration. |
| Tipo: | Artigo |
| URI: | https://hdl.handle.net/1822/33607 |
| ISSN: | 0923-179X |
| e-ISSN: | 1573-8272 |
| Arbitragem científica: | yes |
| Acesso: | Acesso aberto |
| Aparece nas coleções: |
Ficheiros deste registo:
| Ficheiro | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| document_2938_1.pdf | 2,63 MB | Adobe PDF | Ver/Abrir |
