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|Title:||Fermentation pH in stirred tank and air-lift bioreactors affects phytase secretion by Aspergillus japonicus differently but not the particle size|
Silva, Daniel Pereira da
Vicente, A. A.
Teixeira, J. A.
Polizeli, Maria de Lourdes T. M.
|Publisher:||Taylor & Francis|
|Journal:||Biocatalysis and Biotransformation|
|Citation:||Maller, A.; Mota, A.; Silva, D. P.; Vicente, A. A.; Teixeira, J. A.; Polizeli, M. L. T. M., Fermentation pH in stirred tank and air-lift bioreactors affects phytase secretion by Aspergillus japonicus differently but not the particle size. Biocatalysis and Biotransformation, 32(1), 39-44, 2014|
|Abstract(s):||Phytases are mainly produced by filamentous fungi and have great potential for biotechnological use in animal feed treatment, because this enzyme hydrolyzes ester bonds of the phytic acid releasing inositol and inorganic phosphate. The aim of this work was to evaluate the effect of pH on the production of phytase by Aspergillus japonicus in two different bioreactors, known to have different mixing patternsstirred tank and air-lift bioreactors. The maximum phytase production53 U/mLwas obtained at 120 h in the stirred tank while in the air-lift the maximum value was 41 U/mL, observed at 144 h. In fermentations evaluated at controlled pH values (3.5, 6.0, and 7.5), the stirred tank was more efficient for production of phytase than the air-lift. Under these conditions, the highest value was measured at 24 h and pH 3.5. These results were not closely related to fungi particle size, because hyphae with a similar diameter (0.510.63 mm) and sphericity (0.780.87 mm) secreted different amounts of phytase under the conditions studied.|
|Access:||Restricted access (UMinho)|
|Appears in Collections:||CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series|
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