Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/33097

TitleFermentation pH in stirred tank and air-lift bioreactors affects phytase secretion by Aspergillus japonicus differently but not the particle size
Author(s)Maller, Alexandre
Mota, André
Silva, Daniel Pereira da
Vicente, A. A.
Teixeira, J. A.
Polizeli, Maria de Lourdes T. M.
KeywordspHeffect
Phytase
Aspergillus japonicus
Stirred tank
Air-lift
Issue date2014
PublisherTaylor & Francis
JournalBiocatalysis and Biotransformation
CitationMaller, A.; Mota, A.; Silva, D. P.; Vicente, A. A.; Teixeira, J. A.; Polizeli, M. L. T. M., Fermentation pH in stirred tank and air-lift bioreactors affects phytase secretion by Aspergillus japonicus differently but not the particle size. Biocatalysis and Biotransformation, 32(1), 39-44, 2014
Abstract(s)Phytases are mainly produced by filamentous fungi and have great potential for biotechnological use in animal feed treatment, because this enzyme hydrolyzes ester bonds of the phytic acid releasing inositol and inorganic phosphate. The aim of this work was to evaluate the effect of pH on the production of phytase by Aspergillus japonicus in two different bioreactors, known to have different mixing patternsstirred tank and air-lift bioreactors. The maximum phytase production53 U/mLwas obtained at 120 h in the stirred tank while in the air-lift the maximum value was 41 U/mL, observed at 144 h. In fermentations evaluated at controlled pH values (3.5, 6.0, and 7.5), the stirred tank was more efficient for production of phytase than the air-lift. Under these conditions, the highest value was measured at 24 h and pH 3.5. These results were not closely related to fungi particle size, because hyphae with a similar diameter (0.510.63 mm) and sphericity (0.780.87 mm) secreted different amounts of phytase under the conditions studied.
TypeArticle
URIhttp://hdl.handle.net/1822/33097
DOI10.3109/10242422.2013.870559
ISSN1024-2422
1029-2446
e-ISSN1024-2422
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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