Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/33004

TitleChitosan tubes of varying degrees of acetylation for bridging peripheral nerve defects
Author(s)Haastert-Talinia, Kirsten
Geuna, Stefano
Dahlin, Lars B.
Meyer, Cora
Stenberg, Lena
Freier, Thomas
Heimann, Claudia
Barwig, Christina
Pinto, Luis F. V.
Raimondo, Stefania
Gambarotta, Giovanna
Samy, Silvina Maria Ribeiro
Sousa, Nuno
Salgado, A. J.
Ratzka, Andreas
Wrobel, Sandra
Grothe, Claudia
KeywordsChitosan
Nerve guide
Electrophysiology
Histomorphometry
Connective tissue
Degradation
Issue dateDec-2013
PublisherElsevier
JournalBiomaterials
Abstract(s)Biosynthetic nerve grafts are desired as alternative to autologous nerve grafts in peripheral nerve reconstruction. Artificial nerve conduits still have their limitations and are not widely accepted in the clinical setting. Here we report an analysis of fine-tuned chitosan tubes used to reconstruct 10 mm nerve defects in the adult rat. The chitosan tubes displayed low, medium and high degrees of acetylation (DAI: ˜ 2%, DA: ˜ 5%, DAIII: ˜ 20%) and therefore different degradability and microenvironments for the regenerating nerve tissue. Short and long term investigations were performed demonstrating that the chitosan tubes allowed functional and morphological nerve regeneration similar to autologous nerve grafts. Irrespective of the DA growth factor regulation demonstrated to be the same as in controls. Analyses of stereological parameters as well as the immunological tissue response at the implantation site and in the regenerated nerves, revealed that DAI and DAIII chitosan tubes displayed some limitations in the support of axonal regeneration and a high speed of degradation accompanied with low mechanical stability, respectively. The chitosan tubes combine several pre-requisites for a clinical acceptance and DAII chitosan tubes have to be judged as the most supportive for peripheral nerve regeneration.
TypeArticle
URIhttp://hdl.handle.net/1822/33004
DOI10.1016/j.biomaterials.2013.08.074
ISSN0142-9612
1878-5905
Publisher versionwww.elsevier.com
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

Files in This Item:
File Description SizeFormat 
haastert-talini k_biomaterials 2013 posp.pdf20,28 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID