Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/32968

TítuloEvaluation of solid and porous microcarriers for cell growth and production of recombinant proteins
Autor(es)Rodrigues, E.
Costa, A. R.
Henriques, Mariana
Azeredo, Joana
Oliveira, Rosário
Palavras-chaveCell adhesion
Cell proliferation
CultiSpher-S
Cytodex 3
Microcarriers
Optimization
Production
Data2014
EditoraSpringer
RevistaMethods in Molecular Biology
CitaçãoRodrigues, E.; Costa, A. R.; Henriques, Mariana; Azeredo, Joana; Oliveira, Rosário, Evaluation of solid and porous microcarriers for cell growth and production of recombinant proteins. In Pörtner, Ralf, Methods in Molecular Biology, Vol. 1104: Animal Cell Biotechnology - Methods and Protocols (3rd edition), New Jersey: Springer, 2014. ISBN: 978-1-62703-732-7, 137-147
Resumo(s)Microcarrier technology opened new perspectives for anchorage-dependent cell culture, by providing increased surface areas for cell adhesion and proliferation, and therefore improving both cell and product yields obtained in these cultures. The establishment of a successful microcarrier culture depends on many factors, such as the type of microcarrier, the cells, and the culture conditions. In this chapter, the basic steps required for the evaluation and optimization of a microcarrier culture for the purpose of production of recombinant proteins are described, for both solid and porous microcarriers.
Tipoarticle
URIhttp://hdl.handle.net/1822/32968
ISBN9781627037327
DOI10.1007/978-1-62703-733-4_10
ISSN1064-3745
Versão da editorahttp://link.springer.com/book/10.1007/978-1-62703-733-4
Arbitragem científicayes
AcessorestrictedAccess
Aparece nas coleções:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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