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dc.contributor.authorPinheiro, António Mateuspor
dc.contributor.authorPatrício, P.por
dc.contributor.authorAlves, N. D.por
dc.contributor.authorSantos, Ana R. Machadopor
dc.contributor.authorMorais, Mónicapor
dc.contributor.authorBessa, J. M.por
dc.contributor.authorSousa, Nunopor
dc.contributor.authorPinto, Luisapor
dc.description.abstractMeasuring anhedonic behavior in rodents is a challenging task as current methods display only moderate sensitivity to detect anhedonic phenotype and, consequently, results from different labs are frequently incongruent. Herein we present a newly-developed test, the Sweet Drive Test (SDT), which integrates food preference measurement in a non-aversive environment, with ultrasonic vocalizations (USVs) recording. Animals were placed in a soundproofed black arena, under red light illumination, and allowed to choose between regular and sweet food pellets. During the test trials, 50 KHz USVs, previously described to be associated with positive experiences, were recorded. In a first experimental approach, we demonstrate the ability of SDT to accurately characterize anhedonic behavior in animals chronically exposed to stress. In a subsequent set of experiments, we show that this paradigm has high sensitivity to detect mood-improving effects of antidepressants. The combined analysis of both food preference and the number of 50 KHz vocalizations in the SDT provides also a valuable tool to discriminate animals that responded to treatment from non-responder animals.por
dc.description.sponsorshipWe thank S. Borges and A. J. Rodrigues for advice and assistance in the USVs recording procedures and analysis, and H. Almeida and J. J. Cerqueira for comments and suggestions on the experimental design. Furthermore, we thank P. Costa for assistance with 3D reconstruction images. Antonio Mateus-Pinheiro, Patricia Patricio, Monica Morais, and Nuno D. Alves received fellowships from the Portuguese Foundation for Science and Technology (FCT). This study was co-funded by the Life- and Health Sciences Research Institute (ICVS), and ON.2-O NOVO NORTE-North Portugal Regional Operational Programme 2007/2013, of the National Strategic Reference Framework (NSRF) 2007/2013, through the European Regional Development Fund (ERDF).por
dc.publisherFrontiers Mediapor
dc.subjectSweet drive testpor
dc.subjectUltrasonic vocalizationspor
dc.subjectSucrose consumption testpor
dc.titleThe sweet drive test : refining phenotypic characterization of anhedonic behavior in rodentspor
oaire.citationTitleFrontiers in Behavioral Neurosciencepor
dc.subject.wosScience & Technologypor
sdum.journalFrontiers in Behavioral Neurosciencepor
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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