Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/32525

TitleThe sweet drive test : refining phenotypic characterization of anhedonic behavior in rodents
Author(s)Pinheiro, António Mateus
Patrício, P.
Alves, N. D.
Santos, Ana R. Machado
Morais, Mónica
Bessa, J. M.
Sousa, Nuno
Pinto, Luisa
KeywordsDepression
Anhedonia
Sweet drive test
Ultrasonic vocalizations
Antidepressants
Sucrose consumption test
Issue date2014
PublisherFrontiers Media
JournalFrontiers in Behavioral Neuroscience
Abstract(s)Measuring anhedonic behavior in rodents is a challenging task as current methods display only moderate sensitivity to detect anhedonic phenotype and, consequently, results from different labs are frequently incongruent. Herein we present a newly-developed test, the Sweet Drive Test (SDT), which integrates food preference measurement in a non-aversive environment, with ultrasonic vocalizations (USVs) recording. Animals were placed in a soundproofed black arena, under red light illumination, and allowed to choose between regular and sweet food pellets. During the test trials, 50 KHz USVs, previously described to be associated with positive experiences, were recorded. In a first experimental approach, we demonstrate the ability of SDT to accurately characterize anhedonic behavior in animals chronically exposed to stress. In a subsequent set of experiments, we show that this paradigm has high sensitivity to detect mood-improving effects of antidepressants. The combined analysis of both food preference and the number of 50 KHz vocalizations in the SDT provides also a valuable tool to discriminate animals that responded to treatment from non-responder animals.
TypeArticle
URIhttp://hdl.handle.net/1822/32525
DOI10.3389/fnbeh.2014.00074
ISSN1662-5153
Publisher versionhttp://www.frontiersin.org
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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