Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/32453

TítuloDetection of the glucocorticoid receptors in brain protein extracts by SDS-PAGE
Autor(es)Marques, Fernanda
Cerqueira, João
Sousa, João Carlos
Sousa, Nuno
Palavras-chaveGlucocorticoid receptor
Brain
Protein levels
Western blot
Data2014
EditoraSpringer Science
RevistaMethods in Molecular Biology
Resumo(s)Glucocorticoids are steroid hormones vital for organ system homeostasis and for the maintenance of essential biological processes. A significant part of these actions are mediated through glucocorticoid receptor (GR) that belongs to the nuclear receptor superfamily. To cover such variety of processes the different glucocorticoids act through different GR isoforms that are originated due to posttranscriptional and posttranslational mechanisms. For this reason when evaluating the levels of GRs we should preferentially determine protein levels instead of gene expression. Here, we describe the detection by Western blotting of the GR (a and ß isoforms) protein, using macrodissected brain tissue.
Tipoarticle
DescriçãoUncorrected proof
URIhttp://hdl.handle.net/1822/32453
DOI10.1007/978-1-4939-1346-6_20
ISSN1064-3745
Versão da editorahttp://rd.springer.com/protocol/10.1007%2F978-1-4939-1346-6_20
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:ICVS - Artigos em Revistas Internacionais com Referee

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