Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/32449

TitleThe secretome of bone marrow and wharton jelly derived mesenchymal stem cells induces differentiation and neurite outgrowth in Sh-SY5Y cells
Author(s)Pires, Ana O.
Carvalho, Andreia Neves
Sousa, Nuno
Salgado, A. J.
Issue date2014
PublisherHindawi Publishing Corporation
JournalStem Cells International
Abstract(s)The goal of this study was to determine and compare the effects of the secretome of mesenchymal stem cells (MSCs) isolated from human bone-marrow (BMSCs) and the Wharton jelly surrounding the vein and arteries of the umbilical cord (human umbilical cord perivascular cells (HUCPVCs)) on the survival and differentiation of a human neuroblastoma cell line (SH-SY5Y). For this purpose, SH-SY5Y cells were differentiated with conditioned media (CM) from the MSCs populations referred above. Retinoic acid cultured cells were used as control for neuronal differentiated SH-SY5Y cells. SH-SY5Y cells viability assessment revealed that the secretome of BMSCs and HUCPVCs, in the form of CM, was able to induce their survival. Moreover, immunocytochemical experiments showed that CM from both MSCs was capable of inducing neuronal differentiation of SH-SY5Y cells. Finally, neurite lengths assessment and quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) analysis demonstrated that CM from BMSCs and HUCPVCs differently induced neurite outgrowth and mRNA levels of neuronal markers exhibited by SH-SY5Y cells. Overall, our results show that the secretome of both BMSCs and HUCPVCs was capable of supporting SH-SY5Y cells survival and promoting their differentiation towards a neuronal phenotype.
TypeArticle
URIhttp://hdl.handle.net/1822/32449
DOI10.1155/2014/438352
ISSN1687-966X
Publisher versionhttp://www.hindawi.com
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em revistas internacionais / Papers in international journals

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