Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/32198

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dc.contributor.authorRodrigues, Joana Lúcia Lima Correiapor
dc.contributor.authorKluskens, Leonpor
dc.contributor.authorRodrigues, L. R.por
dc.contributor.authorPrather, Kristala L. J.por
dc.date.accessioned2014-12-18T15:14:04Z-
dc.date.available2014-12-18T15:14:04Z-
dc.date.issued2013-11-
dc.identifier.urihttps://hdl.handle.net/1822/32198-
dc.description.abstractCurcuminoids are natural pigments from plants that have been reported as potential cancer-fighting drugs. The aim of this work is to engineer an artificial biosynthetic pathway for curcuminoids production by Escherichia coli. Starting from the substrate tyrosine, the curcumin pathway involves several enzymatic steps: conversion of tyrosine to p-coumaric acid; conversion of p-coumaric acid to caffeic acid; production of caffeoyl-CoA from caffeic acid; production of feruloyl-CoA from caffeoyl-CoA; and finally the production of curcumin from feruloyl-CoA and possibly other curcuminoids, due to enzyme promiscuity. The enzymes involved in the two first enzymatic steps are tyrosine ammonia lyase from Rhodotorula glutinis, P450 CYP199A2 from Rhodopseudomonas palustris, and the redox partners pdr from Pseudomonas putida and pux from R. palustris. These two steps were successfully accomplished. Two CoA ligases from different sources are being explored for the conversion of the different carboxylic acids into their corresponding CoA esters. Different combinations of these enzymes and caffeoyl-CoA 3-methyl transferase may lead to the production of different curcuminoids. For the last step of the pathway two approaches are being studied: the use of diketide-CoA synthase and curcuminoid synthase from Curcuma longa, and curcumin synthase from Oryza sativa that itself catalyzes both steps. Successful construction of the curcuminoids biosynthetic pathway would mark a significant step forward in the in situ production of these poorly soluble, anti-carcinogenic compounds.por
dc.language.isoengpor
dc.rightsopenAccesspor
dc.titleDesign and construction of a new biosynthetic pathway for the production of curcuminoids in Escherichia colipor
dc.typeconferenceAbstract-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.efb-central.org/index.php/syntheticbiology/por
dc.commentsCEB17622por
sdum.publicationstatuspublishedpor
oaire.citationConferenceDate25 - 27 nov. 2013por
sdum.event.typeconferencepor
oaire.citationStartPage1por
oaire.citationEndPage1por
oaire.citationConferencePlaceMálaga, Spainpor
oaire.citationTitle2nd Applied Synthetic Biology in Europepor
dc.date.updated2014-11-28T17:56:57Z-
Aparece nas coleções:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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