Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/31998

TitleModulation of crude glycerol fermentation byClostridium pasteurianum DSM 525 towards theproduction of butanol
Author(s)Gallardo, R.
Alves, M. M.
Rodrigues, L. R.
KeywordsButanol
Clostridium pasteurianum
Crude glycerol
Butyrate
1,3-Propanediol
Issue date2014
PublisherPergamon Press Ltd.
JournalBiomass and Bioenergy
Abstract(s)High production yields and productivities are requisites for the development of an industrial butanol production process based on biodiesel-derived crude glycerol. However, impurities present in this substrate and/or the concentration of glycerol itself can affect the microbial metabolism. In this work, the effect of crude glycerol concentration on the production of butanol and 1,3-propanediol (1,3-PDO) by Clostridium pasteurianum DSM 525 is studied. Also, the effect of acetate and butyrate supplementation to the culture medium and the culture medium composition are evaluated. The results showed a marked effect of crude glycerol concentration on the product yield. The competitive nature of butanol and 1,3-PDO pathways has been evident, and a shift to the butanol pathway once using higher substrate concentrations (up to 35 g l 1) was clearly observed. Butyrate supplementation to the culture medium resulted in a 45% higher butanol titre, a lower production of 1,3-PDO and it decreased the fermentation time. Acetate supplementation also increased the butanol titre but the fermentation was longer. Even though glycerol consumption could not be increased over 32 g l 1, when the concentrations of NH4Cl and FeCl2 were simultaneously increased, the results obtained were similar to those observed when butyrate was supplemented to the culture medium; a 35% higher butanol yield at the expense of 1,3-PDO and a shorter fermentation. The results herein gathered suggest that there are other factors besides butanol inhibition and nutrient limitation that affect the glycerol consumption.
TypeArticle
URIhttp://hdl.handle.net/1822/31998
DOI10.1016/j.biombioe.2014.10.015
ISSN0961-9534
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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