Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/31891

TitleGlycosylation: impact, control and improvement during therapeutic protein production
Author(s)Costa, A. R.
Rodrigues, E.
Henriques, Mariana
Oliveira, Rosário
Azeredo, Joana
KeywordsAnalytical methodologies
Culture parameters
Expression system
Glycoengineering
Mammalian cells
Process development
Issue date2014
PublisherInforma Healthcare
JournalCritical Reviews in Biotechnology
Abstract(s)The emergence of the biopharmaceutical industry represented a major revolution for modern medicine, through the development of recombinant therapeutic proteins that brought new hope for many patients with previously untreatable diseases. The ever-growing demand for these therapeutics forces a constant technological evolution to increase product yields while simultaneously reducing costs. However, the process changes made for this purpose may also affect the quality of the product, a factor that was initially overlooked but which is now a major focus of concern. Of the many properties determining product quality, glycosylation is regarded as one of the most important, influencing, for example, the biological activity, serum half-life and immunogenicity of the protein. Consequently, monitoring and control of glycosylation is now critical in biopharmaceutical manufacturing and a requirement of regulatory agencies. A rapid evolution is being observed in this context, concerning the influence of glycosylation in the efficacy of different therapeutic proteins, the impact on glycosylation of a diversity of parameters/processes involved in therapeutic protein production, the analytical methodologies employed for glycosylation monitoring and control, as well as strategies that are being explored to use this property to improve therapeutic protein efficacy (glycoengineering). The present work reviews the main findings on these subjects, providing an up-to-date source of information to support further studies.
TypeArticle
URIhttp://hdl.handle.net/1822/31891
DOI10.3109/07388551.2013.793649
ISSN0738-8551
e-ISSN1549-7801
Publisher versionhttp://informahealthcare.com/journal/bty
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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