Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/31883

TitleApplication of MALDI-TOF MS for requalification of a Candida clinical isolates culture collection
Author(s)Lima-Neto, Reginaldo
Santos, Cledir
Lima, Nelson
Sampaio, Paula
Pais, Célia
Neves, Rejane P.
KeywordsBRCs
Candida
Clinical yeasts
Culture collections
MALDI-TOF MS
Issue date2014
PublisherSociedade Brasileira de Microbiologia
JournalBrazilian Journal of Microbiology
Abstract(s)Microbial culture collections underpin biotechnology applications and are important resources for clinical microbiology by supplying reference strains and/or performing microbial identifications as a service. Proteomic profiles by MALDI-TOF MS have been used for Candida spp. identification in clinical laboratories and demonstrated to be a fast and reliable technique for the routine identification of pathogenic yeasts. The main aim of this study was to apply MALDI-TOF MS combined with classical phenotypic and molecular approaches to identify Candida clinical isolates preserved from 1 up to 52 years in a Brazilian culture collection and assess its value for the identification of yeasts preserved in this type of collections. Forty Candida spp. clinical isolates were identified by morphological and biochemical analyses. Identifications were also performed by the new proteomic approach based on MALDI-TOF MS. Results demonstrated 15% discordance when compared with morphological and biochemical analyses. Discordant isolates were analysed by ITS sequencing, which confirmed the MALDI-TOF MS identifications and these strains were renamed in the culture collection catalogue. In conclusion, proteomic profiles by MALDI-TOF MS represents a rapid and reliable method for identifying clinical Candida species preserved in culture collections and may present clear benefits when compared with the performance of existing daily routine methods applied at health centres and hospitals.
TypeArticle
URIhttp://hdl.handle.net/1822/31883
DOI10.1590/S1517-83822014005000044
ISSN1517-8382
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series
DBio - Artigos/Papers

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