Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/31734

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dc.contributor.authorOliveira, Carla Cristina Marques depor
dc.contributor.authorTeixeira, J. A.por
dc.contributor.authorDomingues, Lucíliapor
dc.date.accessioned2014-12-09T14:36:45Z-
dc.date.available2014-12-09T14:36:45Z-
dc.date.issued2014-
dc.identifier.issn1664-462Xpor
dc.identifier.urihttp://hdl.handle.net/1822/31734-
dc.description.abstractFrutalin is a homotetrameric partly-glycosylated alpha-D-galactose-binding lectin of biomedical interest from Artocarpus incisa (breadfruit) seeds, belonging to the jacalin-related lectins family. As other plant lectins, frutalin is a heterogeneous mixture of several isoforms possibly with distinct biological activities. The main problem of using such lectins as biomedical tools is that “batch-to-batch” variation in isoforms content may lead to inconstant results. The production of lectins by recombinant means has the advantage of obtaining high amounts of proteins with defined amino-acid sequences and more precise properties. In this mini review, we provide the strategies followed to produce two different forms of frutalin in two different microbial systems: Escherichia coli and Pichia pastoris. The processing and functional properties of the recombinant frutalin obtained from these hosts are compared to those of frutalin extracted from breadfruit. Emphasis is given particularly to recombinant frutalin produced in P. pastoris, which showed a remarkable capacity as biomarker of human prostate cancer and as apoptosis-inducer of cancer cells. Recombinant frutalin production opens perspectives for its development as a new tool in human medicine.por
dc.description.sponsorship(undefined)por
dc.language.isoengpor
dc.publisherFrontiers Mediapor
dc.relationinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/129208/PT-
dc.rightsopenAccesspor
dc.subjectRecombinant frutalinpor
dc.subjectLectin isoformspor
dc.subjectPichia pastoris expression systempor
dc.subjectGlycosylationpor
dc.subjectEscherichia coli expression systempor
dc.subjectBiomedical applicationpor
dc.subjectTumor biomarkerpor
dc.subjectApoptosis-inducerpor
dc.titleRecombinant production of plant lectins in microbial systems for biomedical application – the frutalin case studypor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.frontiersin.org/Plant_Physiologypor
dc.commentsCEB17934por
sdum.publicationstatuspublishedpor
oaire.citationStartPage1por
oaire.citationEndPage6por
oaire.citationIssue390por
oaire.citationTitleFrontiers in plant sciencepor
oaire.citationVolume5por
dc.date.updated2014-12-08T16:01:19Z-
dc.identifier.essn1664-462X-
dc.identifier.doi10.3389/fpls.2014.00390por
sdum.journalFrontiers in plant sciencepor
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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