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TitleHSA nanocapsules functionalized with monoclonal antibodies for targeted drug delivery
Author(s)Rollett, Alexandra
Reiter, Tamara
Ohradanova-Repic, Anna
Machacek, Christian
Paulo, Artur Cavaco
Stockinger, Hannes
Guebitz, G. M.
Drug delivery
Albumin nanocapsules
Cross linking
Issue date2013
JournalInternational Journal of Pharmaceutics
CitationRollett, A.; Reiter, T.; Ohradanova-Repic, A.; Machacek, C.; Paulo, Artur Cavaco; Stockinger, H.; Guebitz, G. M., HSA nanocapsules functionalized with monoclonal antibodies for targeted drug delivery. International Journal of Pharmaceutics, 458(1), 1-8, 2013
Abstract(s)The chronic autoimmune disorder rheumatoid arthritis (RA) affects millions of adults and children every year. Chronically activated macrophages secreting enzymes and inflammatory cytokines play a key role in RA. Distinctive marker molecules on the macrophage surface could be used to design a targeted drug delivery device for the treatment of RA without affecting healthy cells and tissues. Here, different methods for covalent attachment of antibodies (mAb) recognizing MHC class II molecules found on macrophages onto human serum albumin (HSA) nanocapsules were compared. HSA nanocapsules were prepared with a hydrodynamic diameter of 500.7 ± 9.4 nm and a narrow size distribution as indicated by a polydispersity index (PDI) of 0.255 ± 0.024. This was achieved by using a sonochemical process avoiding toxic cross linking agents and emulsifiers. Covalent binding of mAb on the surface of HSA nanocapsules was realized using polyethyleneglycol (PEG)3000 as spacer molecule. The presence of mAb was confirmed by confocal laser scanning microscopy (CLSM) and enzyme-linked immunosorbent assay (ELISA). Specific binding of mAb-HSA nanocapsules to MHC class II molecules on antigen-presenting cells was demonstrated by flow cytometry analysis.
AccessRestricted access (UMinho)
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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