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TitleInfluence of culture media on the physical and chemical properties of Ag–TiCN coatings
Author(s)Carvalho, I.
Escobar Galindo, R.
Henriques, Mariana
Palacio, C.
Carvalho, S.
KeywordsAg coatings
Microbial and cell culture media
Issue date2014
PublisherIOP Publishing
JournalJournal of Physics D: Applied Physics
Abstract(s)The aim of this study was to verify the possible physical and chemical changes that may occur on the surface of Ag–TiCN coatings after exposure to the culture media used in microbiological and cytotoxic assays, respectively tryptic soy broth (TSB) and Dulbecco's modified eagle's medium (DMEM). After sample immersion for 24 h in the media, analyses were performed by glow discharge optical emission spectroscopy discharge radiation (GDOES), Rutherford backscattering spectroscopy (RBS) and x-ray photoelectron spectroscopy (XPS). The results of GDOES profile, RBS and XPS spectra, of samples immersed in TSB, demonstrated the formation of a thin layer of carbon, oxygen and nitrogen that could be due to the presence of proteins in TSB. After 24 h of immersion in DMEM, the results showed the formation of a thin layer of calcium phosphates on the surface, since the coatings displayed a highly oxidized surface in which calcium and phosphorus were detected. All these results suggested that the formation of a layer on the coating surface prevented the release of silver ions in concentrations that allow antibacterial activity.
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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