1. Universidade do Minho
  2. Escola de Engenharia | School of Engineering
  3. Centro de Engenharia Biológica | Centre of Biological Engineering
  4. CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series
Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/31659

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Campo DCValorIdioma
dc.contributor.authorAguiar, Tatiana Quintapor
dc.contributor.authorDinis, Cláudiapor
dc.contributor.authorDomingues, Lucíliapor
dc.date.accessioned2014-12-05T12:02:51Z-
dc.date.available2014-12-05T12:02:51Z-
dc.date.issued2014-
dc.identifier.citationAguiar, T. Q.; Dinis, C.; Domingues, Lucília, Cre-loxP-based system for removal and reuse of selection markers in Ashbya gossypii targeted engineering. Fungal Genetics and Biology, 68, 1-8, 2014por
dc.identifier.issn1087-1845por
dc.identifier.urihttps://hdl.handle.net/1822/31659-
dc.description.abstractThe filamentous ascomycete Ashbya gossypii is amenable to genetic manipulation and is an excellent model system for studying eukaryotic cell biology. However, the number of selection markers in current use for both targeted gene integration and disruption in this fungus are very limited. Therefore, the Cre-loxP recombination system was adapted for use in A. gossypii and its effectiveness in recycling marker genes was demonstrated by constructing both single and double deleted Agura3 and Agade1 auxotrophic strains free of exogenous markers. In spite of its wide use, this is the first report in which the Cre-loxP system was applied to A. gossypii, opening new perspectives for targeted engineering of this fungus with several promising biotechnological applications.por
dc.description.sponsorshipThis work was supported by Fundacao para a Ciencia e a Tecnologia, Portugal, through Project AshByofactory (PTDC/EBB-EBI/101985/2008 - FCOMP-01-0124-FEDER-009701), Project RECI/BBB-EBI/0179/2012 - FCOMP-01-0124-FEDER-027462, Strategic Project PEst-OE/EQB/LA0023/2013, Project BioInd (NORTE-07-0124-FEDER-000028) co-funded by the Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN, FEDER, and MIT-Portugal Program PhD Grant SFRH/BD/39112/2007 to Tatiana Q. Aguiar.por
dc.language.isoengpor
dc.publisherAcademic Presspor
dc.relationinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/126270/PT-
dc.rightsopenAccesspor
dc.subjectAshbya gossypiipor
dc.subjectCre-loxP systempor
dc.subjectGene targetingpor
dc.subjectMarker recyclingpor
dc.subjectRecombinase expressionpor
dc.titleCre-loxP-based system for removal and reuse of selection markers in Ashbya gossypii targeted engineeringpor
dc.typearticle-
dc.peerreviewedyespor
dc.commentsCEB17743por
sdum.publicationstatuspublishedpor
oaire.citationStartPage1por
oaire.citationEndPage8por
oaire.citationConferencePlaceUnited States-
oaire.citationTitleFungal Genetics and Biologypor
oaire.citationVolume68por
dc.date.updated2014-11-28T17:54:51Z-
dc.identifier.eissn1096-0937-
dc.identifier.doi10.1016/j.fgb.2014.04.009por
dc.identifier.pmid24792968por
dc.subject.wosScience & Technologypor
sdum.journalFungal Genetics and Biologypor
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