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|Title:||Immobilization of lectin preparations from Moringa oleifera seeds in inert supports for water purification|
|Author(s):||Santos, Andréa F. S.|
Luz, L. A.
Cunha, M. G. C.
Brito, A. G.
Paiva, Patrícia M. G.
Teixeira, J. A.
Coelho, L. C. B. B.
|Publisher:||Universidade do Minho. Departamento de Engenharia Biológica (DEB)|
|Citation:||Santos, Andréa F. S.; Luz, L. A.; Carneiro-da-Cunha, M. G.; Brito, A. G.; Paiva, P. M. G.; Teixeira, J. A.; Coelho, L. C. B. B.; Nogueira, R., Immobilization of lectin preparations from Moringa oleifera seeds in inert supports for water purification. VALNATURA - A Europe-Latin América post-graduate research network in the VALorization of NATURAL resources, 2008. ISBN: 978-972-97810-5-6, 106-113|
|Abstract(s):||Humic acids are linked to the formation of carcinogenic disinfection by-products upon chlorination of drinking water. In this work the first focus was tocharacterize the affinity of protein preparations obtained from Moringa oleifera seeds (extract, E, fraction, 0-60F and M.oleifera lectin, MoL) to bind humic acids. The second focus was to select a suitable support to immobilize MoL and to assess humic acid removal from water in a packed bed column. Specific hemagglutinating activity (SHA) decreased by 94 % for both E and MoL and by 50 % for 0-60F in the presence of a commercial humic acid. Humic acid-MoL precipitation bands were observed in the diffusion gel. Both results indicate humic acid-lectin binding. Carbohydrates, potassium and calcium ions as well as pH va-lues affected the SHA of MoL. Ahumic acid removal of 30.4 mg/g (expressed as mass of humic acid per mass of support) was obtained in a column packed with sepharose immobilized MoL receiving a 20 mg/L of carbon humic acid solution.|
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