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TitleImmobilization of lectin preparations from Moringa oleifera seeds in inert supports for water purification
Author(s)Santos, Andréa F. S.
Luz, L. A.
Cunha, M. G. C.
Brito, A. G.
Paiva, Patrícia M. G.
Teixeira, J. A.
Coelho, L. C. B. B.
Nogueira, R.
Moringa oleifera
Humic acid
Water treatment
Issue date2008
PublisherUniversidade do Minho. Departamento de Engenharia Biológica (DEB)
CitationSantos, Andréa F. S.; Luz, L. A.; Carneiro-da-Cunha, M. G.; Brito, A. G.; Paiva, P. M. G.; Teixeira, J. A.; Coelho, L. C. B. B.; Nogueira, R., Immobilization of lectin preparations from Moringa oleifera seeds in inert supports for water purification. VALNATURA - A Europe-Latin América post-graduate research network in the VALorization of NATURAL resources, 2008. ISBN: 978-972-97810-5-6, 106-113
Abstract(s)Humic acids are linked to the formation of carcinogenic disinfection by-products upon chlorination of drinking water. In this work the first focus was tocharacterize the affinity of protein preparations obtained from Moringa oleifera seeds (extract, E, fraction, 0-60F and M.oleifera lectin, MoL) to bind humic acids. The second focus was to select a suitable support to immobilize MoL and to assess humic acid removal from water in a packed bed column. Specific hemagglutinating activity (SHA) decreased by 94 % for both E and MoL and by 50 % for 0-60F in the presence of a commercial humic acid. Humic acid-MoL precipitation bands were observed in the diffusion gel. Both results indicate humic acid-lectin binding. Carbohydrates, potassium and calcium ions as well as pH va-lues affected the SHA of MoL. Ahumic acid removal of 30.4 mg/g (expressed as mass of humic acid per mass of support) was obtained in a column packed with sepharose immobilized MoL receiving a 20 mg/L of carbon humic acid solution.
TypeBook part
AccessOpen access
Appears in Collections:CEB - Livros e Capítulos de Livros / Books and Book Chapters

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