Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/31582

TitleRapid detection of Ganoderma-infected oil palms by microwave ergosterol extraction with HPLC and TLC
Author(s)Muniroh, M. S.
Sariah, M.
Abidin, M. A. Zainal
Lima, Nelson
Paterson, R. R. M.
KeywordsMicrowave assisted extraction
Ganoderma
Ergosterol
Oil palm
TLC
HPLC
Issue date2014
PublisherElsevier
JournalJournal of microbiological methods
Abstract(s)Detection of basal stem rot (BSR) by Ganoderma of oil palms was based on foliar symptoms and production of basidiomata. Enzyme-Linked Immunosorbent Assays-Polyclonal Antibody (ELISA-PAB) and PCR have been proposed as early detection methods for the disease. These techniques are complex, time consuming and have accuracy limitations. An ergosterol method was developed which correlated well with the degree of infection in oil palms, including samples growing in plantations. However, the method was capable of being optimised. This current study was designed to develop a simpler, more rapid and efficient ergosterol method with utility in the field that involved the use of microwave extraction. The optimised procedure involved extracting a small amount of Ganoderma, or Ganoderma-infected oil palm suspended in low volumes of solvent followed by irradiation in a conventional microwave oven at 70 °C and medium high power for 30 s, resulting in simultaneous extraction and saponification. Ergosterol was detected by thin layer chromatography (TLC) and quantified using high performance liquid chromatography with diode array detection. The TLC method was novel and provided a simple, inexpensive method with utility in the field. The new method was particularly effective at extracting high yields of ergosterol from infected oil palm and enables rapid analysis of field samples on site, allowing infected oil palms to be treated or culled very rapidly. Some limitations of the method are discussed herein. The procedures lend themselves to controlling the disease more effectively and allowing more effective use of land currently employed to grow oil palms, thereby reducing pressure to develop new plantations.
TypeArticle
URIhttp://hdl.handle.net/1822/31582
DOI10.1016/j.mimet.2014.03.005
ISSN0167-7012
e-ISSN0167-7012
Publisher versionwww.elsevier.com/locate/jmicmeth
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
document_17754_1.pdf409,56 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID