Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/31455

TitleProduction and characterization of protease from Penicillium aurantiogriseum URM 4622
Author(s)Rodrigues, Priscila M. B.
Lima, Carolina A.
Filho, José Luiz L.
Teixeira, J. A.
Porto, Ana L. F.
Cunha, Maria G. Carneiro da
KeywordsPenicillium aurantiogriseum
Production
Characterization
Protease
Bacth
Bioreactor
Detergent
Issue date2008
CitationRodrigues, Priscila M. B.; Lima, Carolina; Filho, J. L. Lima; Teixeira, José A.; Porto, A. L. F.; Carneiro-da-Cunha, M. G., Production and characterization of protease from Penicillium aurantiogriseum URM 4622. VALNATURA - A Europe-Latin América post-graduate research network in the VALorization of NATURAL resources, 2008. ISBN: 978-972-97810-5-6, 97-104
Abstract(s)Proteases with new properties are required due to their increasing industrial importance. In this work, the optimal fermentation conditions for the production of a protease from Penicillium aurantiogriseum dierchx (URM-4622) are presented together with partial characterization of the protease catalytic properties. The batch fermentation conditions that allow for the highest specific proteolytic activity are 26 ºC, pH 7.0, and 25 % saturation dissolved O2 concentration. The obtained protease is stable over a wide range of pH (5.8 to 9.5) and temperature (25 to 40 ºC) values. In the presence of Zn2+ a 26 % reduction in the enzyme proteolytic activity occurs and, in contrast, Mn2+ enhances its activity by 28.9 %. 96.2 % and 70.8 % of the protease activity are maintained after 90 min incubation in 5 and 10 % (v/v) H2O2 aqueous solutions, respectively. PMSF inhibition reveals that this enzyme is a serine protease. Protease is able to hydrolyze different proteins.
TypeBook part
URIhttp://hdl.handle.net/1822/31455
ISBN978-972-97810-5-6
AccessOpen access
Appears in Collections:CEB - Livros e Capítulos de Livros / Books and Book Chapters

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