Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/31443

TitleEnzymatic phosphorylation of silk fibroins : a platform for the production of biocompatible, cell-static, materials
Author(s)Volkov, Vadim
Vasconcelos, Andreia
Sárria, M. P.
Gomes, A. C.
Paulo, Artur Cavaco
Issue date25-May-2014
CitationVolkov, V.; Vasconcelos, Andreia; Sárria, M. P.; Gomes, A. C.; Paulo, Artur Cavaco, Enzymatic phosphorylation of silk fibroins: a platform for the production of biocompatible, cell-static, materials. IPFB2014 - 8th International Conference on Polymer and Fiber Biotechnology. Braga, Portugal, May 25-27, 2014.
Abstract(s)Silks are natural protein polymers produced by insects. Silk heavy chain of B.mori is primarily composed of hydrophobic, –(–Ala–Gly–)n– -sheet crystalline domains. Based on silk biocompatibility, biodegradability and strength, different materials were developed. Silk offers a stabilizing environment for incorporated proteins and molecules. Silk properties can be controlled via structure manipulation, by coupling molecules of biological significance; its Tyr and Ser residues can be modified. Once incorporated into a protein, the phosphate group establishes hydrogen bonds that affect intra- and inter-molecular interactions16. Phosphorylation is stable under physiological conditions, thus directing the formation and reorganization of protein networks. Curiously, using phosphorylation for protein functionalization is largely unexplored. Significant research is devoted to bio-inspired materials with various cell-differentiating and cell-supporting features. However, little attention is paid to develop cell-static bio-materials. Such materials do not promote cell growth. That can be achieved by lowering the probability of cell attachment to the material, via creation of negatively charged material surface. The goal of this study was to produce bio-compatible materials with the cell-static properties by phosphorylation. Silk solutions were made to cast films of variable pH and phosphorylated content. Obtained materials were tested and a dependency between amount of phosphorylation and bio-chemical properties confirmed.
TypeAbstract
URIhttp://hdl.handle.net/1822/31443
Publisher versionhttp://ipfb2014.wix.com/ipfb2014
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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