Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/31120

TítuloDifferential regulation of osteogenic differentiation of stem cells on surface roughness gradients
Autor(es)Torres, Ana B. Faia
Lischer, Stefanie Guimond
Rottmar, Markus
Charnley, Mirren
Goren, T.
Maniura-Weber, Katharina
Spencer, Nicholas D.
Reis, R. L.
Textor, Marcus
Neves, N. M.
Palavras-chaveMesenchymal stem cell
Osteogenesis
Polycaprolactone
Surface roughness
DataAgo-2014
EditoraElsevier
RevistaBiomaterials
CitaçãoFaia-Torres A. B., Guimond-Lischer S., Rottmar M., Charnley M., Goren T., Maniura-Weber K., Spencer N. D., Reis R. L., Textor M., Neves N. M. Differential regulation of osteogenic differentiation of stem cells on surface roughness gradients, Biomaterials, Vol. 35, Issue 33, pp. 9023-9032, doi:10.1016/j.biomaterials.2014.07.015, 2014
Resumo(s)Tissue engineering using scaffold-cell constructs holds the potential to develop functional strategies to regenerate bone. The interface of orthopedic implants with the host tissues is of great importance for its later performance. Thus, the optimization of the implant surface in a way that could stimulate osteogenic differentiation of mesenchymal stem cells (MSCs) is of significant therapeutic interest. The effect of surface roughness of polycaprolactone (PCL) on the osteogenic differentiation of human bone-marrow MSCs was investigated. We prepared surface roughness gradients of average roughness (Ra) varying from the sub-micron to the micrometer range (~0.5e4.7 mm), and mean distance between peaks (RSm) gradually varying from ~214 mm to 33 mm. We analyzed the degree of cytoskeleton spreading, expression of alkaline phosphatase, collagen type 1 and mineralization. The response of cells to roughness divided the gradient into three groups of elicited stem cell behavior: 1) faster osteogenic commitment and strongest osteogenic expression; 2) slower osteogenic commitment but strong osteogenic expression, and 3) similar or inferior osteogenic potential in comparison to the control material. The stem-cell modulation by specific PCL roughness surfaces highlights the potential for creating effective solutions for orthopedic applications featuring a clinically relevant biodegradable material.
Tipoarticle
URIhttp://hdl.handle.net/1822/31120
DOI10.1016/j.biomaterials.2014.07.015
Versão da editorahttp://www.sciencedirect.com/science/article/pii/S0142961214008096
Arbitragem científicayes
AcessorestrictedAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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