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https://hdl.handle.net/1822/30885
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Campo DC | Valor | Idioma |
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dc.contributor.author | Susano, Maria A. | por |
dc.contributor.author | Leonor, I. B. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Azevedo, Helena S. | por |
dc.date.accessioned | 2014-11-13T11:38:59Z | - |
dc.date.available | 2014-11-13T11:38:59Z | - |
dc.date.issued | 2014-02 | - |
dc.date.submitted | 2014-02 | - |
dc.identifier.issn | 0021-8995 | por |
dc.identifier.uri | https://hdl.handle.net/1822/30885 | - |
dc.description.abstract | The fabrication of a biomaterial scaffold, with adequate physical and structural properties for tissue engineering applica- tions, is reported. A blend of starch with ethylene-vinyl alcohol (50/50 w/w, SEVA-C) is used to produce 3D fibre-mesh scaffolds by wet-spinning. The scaffolds are characterized in terms of morphology, porosity, interconnectivity, and pore size, using scanning elec- tron microscopy (SEM) and microcomputed tomography (µCT). The degradation behavior, as well as the mechanical properties of the scaffolds, is investigated in presence of alpha-amylase enzyme at physiological concentration. Scaffolds with porosities ranging from 43 to 52%, interconnectivity of ≈70.5% and pore size between 118 and 159 µm, can be fabricated using the proposed methodology. The scaffolds exhibit an elastic behavior in the wet state with a compressive modulus of 7.96 ±0.32 MPa. Degradation studies show that SEVA-C scaffolds are susceptible to enzymatic degradation by alpha-amylase, confirmed by the increase of weight loss (40% of weight loss after 12 weeks) and presence of degradation products (reducing sugars) in solution. The diameter of SEVA-C scaffolds decreases with degradation time, increasing the overall porosity, interconnectivity and pore size. In vitro cell studies with human osteosarcoma cell line (SaOs-2) showed a nontoxic and cytocompatible behavior of the developed fibre mesh scaffolds. The positive cellular response, together with structural and degradable properties, suggests that 3D SEVA-C fibre-meshes may be good candidates as tissue engineering scaffolds. | por |
dc.description.sponsorship | This work was supported by national funds through the Portuguese Foundation for Science and Technology under the scope of the project PTDC/CTM/67560/2006 and by the European Regional Development Fund (ERDF) through the Operational Competitiveness Programme "COMPETE" (FCOMP-01-0124-FEDER007148). The authors thank Dr Emanuel Fernandes of the 3B's Research Group at the University of Minho for his assistance analyzing the data from mechanical tests. | por |
dc.language.iso | eng | por |
dc.publisher | John Wiley and Sons | por |
dc.rights | restrictedAccess | por |
dc.subject | Biodegradable | por |
dc.subject | Biomaterials | por |
dc.subject | Elastomers | por |
dc.subject | Mechanical properties | por |
dc.subject | Porous materials | por |
dc.title | Elastic biodegradable starch/ethylene-co-vinyl alcohol fibre-mesh scaffolds for tissue engineering applications | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1002/app.40504/abstract | por |
dc.comments | http://www.3bs.uminho.pt/node/17905 | por |
sdum.publicationstatus | published | por |
oaire.citationIssue | 14 | por |
oaire.citationTitle | Journal of Applied Polymer Science | por |
oaire.citationVolume | 131 | por |
dc.date.updated | 2014-11-05T11:23:45Z | - |
dc.identifier.doi | 10.1002/app.40504 | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Applied Polymer Science | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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17905-MSusano_JApplPolymSci2014_OnlinePublication.pdf Acesso restrito! | 681,44 kB | Adobe PDF | Ver/Abrir |