Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/30885

TitleElastic biodegradable starch/ethylene-co-vinyl alcohol fibre-mesh scaffolds for tissue engineering applications
Author(s)Susano, Maria A.
Leonor, I. B.
Reis, R. L.
Azevedo, Helena S.
KeywordsBiodegradable
Biomaterials
Elastomers
Mechanical properties
Porous materials
Issue dateFeb-2014
PublisherJohn Wiley and Sons
JournalJournal of Applied Polymer Science
Abstract(s)The fabrication of a biomaterial scaffold, with adequate physical and structural properties for tissue engineering applica- tions, is reported. A blend of starch with ethylene-vinyl alcohol (50/50 w/w, SEVA-C) is used to produce 3D fibre-mesh scaffolds by wet-spinning. The scaffolds are characterized in terms of morphology, porosity, interconnectivity, and pore size, using scanning elec- tron microscopy (SEM) and microcomputed tomography (µCT). The degradation behavior, as well as the mechanical properties of the scaffolds, is investigated in presence of alpha-amylase enzyme at physiological concentration. Scaffolds with porosities ranging from 43 to 52%, interconnectivity of ≈70.5% and pore size between 118 and 159 µm, can be fabricated using the proposed methodology. The scaffolds exhibit an elastic behavior in the wet state with a compressive modulus of 7.96 ±0.32 MPa. Degradation studies show that SEVA-C scaffolds are susceptible to enzymatic degradation by alpha-amylase, confirmed by the increase of weight loss (40% of weight loss after 12 weeks) and presence of degradation products (reducing sugars) in solution. The diameter of SEVA-C scaffolds decreases with degradation time, increasing the overall porosity, interconnectivity and pore size. In vitro cell studies with human osteosarcoma cell line (SaOs-2) showed a nontoxic and cytocompatible behavior of the developed fibre mesh scaffolds. The positive cellular response, together with structural and degradable properties, suggests that 3D SEVA-C fibre-meshes may be good candidates as tissue engineering scaffolds.
TypeArticle
URIhttp://hdl.handle.net/1822/30885
DOI10.1002/app.40504
ISSN0021-8995
Publisher versionhttp://onlinelibrary.wiley.com/doi/10.1002/app.40504/abstract
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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