Please use this identifier to cite or link to this item:

TitleData from the manuscript [A role of supraspinal galanin in behavioural hyperalgesia in the rat]
Author(s)Amorim, Diana
David-Pereira, Ana
Marques, Patrícia
Puga, Sónia Andreia Silva
Rebelo, Patrícia
Costa, Patrício Soares
Pertovaara, Antti
Almeida, Armando
Pinto-Ribeiro, Filipa
Keywordsexperimental monoarthritis
pronociception, galanin
galanin receptor type-1
dorsomedial hypothalamic nucleus
rostral ventromedial medulla
Issue date2014
Abstract(s)In chronic pain disorders, galanin (GAL) is able to either facilitate or inhibit nociception in the spinal cord but the contribution of supraspinal galanin to pain signalling is mostly unknown. The dorsomedial nucleus of the hypothalamus (DMH) is rich in galanin receptors (GALR) and is involved in behavioural hyperalgesia. In this study, we evaluated the contribution of supraspinal GAL to behavioural hyperalgesia in experimental monoarthritis. In Wistar-Han males with a four week kaolin/carrageenan-induced monoarthritis (ARTH), paw-withdrawal latency (PWL) was assessed before and after DMH administration of exogenous GAL, a non-specific GALR antagonist (M40), a specific GALR1 agonist (M617) and a specific GALR2 antagonist (M871). Additionally, the analysis of c-Fos expression after GAL injection in the DMH was used to investigate the potential involvement of brainstem pain control centres. Finally, electrophysiological recordings were performed to evaluate whether pronociceptive On- or antinociceptive Off-like cells in the rostral ventromedial medulla (RVM) relay the effect of GAL. Exogenous GAL in the DMH decreased PWL in ARTH and SHAM animals, an effect that was mimicked by a GALR1 agonist (M617). In SHAM animals, an unselective GALR antagonist (M40) increased PWL, while a GALR2 antagonist (M871) decreased PWL. M40 or M871 failed to influence PWL in ARTH animals. Exogenous GAL increased c-Fos expression in the RVM and dorsal raphe nucleus (DRN), with effects being more prominent in SHAM than ARTH animals. Exogenous GAL failed to influence activity of RVM On- or Off-like cells of SHAM and ARTH animals. Overall, exogenous GAL in the DMH had a pronociceptive effect that is mediated by GALR1 in healthy and arthritic animals and is associated with alterations of c-Fos expression in RVM and DRN that are serotonergic brainstem nuclei known to be involved in the regulation of pain.
DescriptionManuscript: "A role of supraspinal galanin in behavioural hyperalgesia in the rat" – by Amorim et al, PLoS One. Available at:
AccessOpen access
Appears in Collections:ICVS - Dados científicos / Research data

Files in This Item:
File Description SizeFormat 
AmorimD_PLoSOne_Galanin_Data.xlsxResearch data179,86 kBUnknownView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID