Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/30426

TítuloAutomated image analysis of lung branching morphogenesis from microscopic images of fetal rat explants
Autor(es)Rodrigues, Pedro L.
Rodrigues, Nuno F.
Duque, Duarte
Granja, Sara Costa
Pinto, Jorge Correia
Vilaça, João L.
Data2014
EditoraHindawi Publishing Corporation
RevistaComputational and mathematical methods in medicine
Resumo(s)Background. Regulating mechanisms of branching morphogenesis of fetal lung rat explants have been an essential tool for molecular research. This work presents a new methodology to accurately quantify the epithelial, outer contour, and peripheral airway buds of lung explants during cellular development from microscopic images. Methods. The outer contour was defined using an adaptive and multiscale threshold algorithm whose level was automatically calculated based on an entropy maximization criterion. The inner lung epithelium was defined by a clustering procedure that groups small image regions according to the minimum description length principle and local statistical properties. Finally, the number of peripheral buds was counted as the skeleton branched ends from a skeletonized image of the lung inner epithelia. Results. The time for lung branching morphometric analysis was reduced in 98% in contrast to the manual method. Best results were obtained in the first two days of cellular development, with lesser standard deviations. Nonsignificant differences were found between the automatic and manual results in all culture days. Conclusions. The proposed method introduces a series of advantages related to its intuitive use and accuracy, making the technique suitable to images with different lighting characteristics and allowing a reliable comparison between different researchers.
Tipoarticle
DescriçãoArticle ID 820214
URIhttp://hdl.handle.net/1822/30426
DOI10.1155/2014/820214
ISSN1748-670X
1748-6718
Versão da editorahttp://www.hindawi.com/journals/cmmm/2014/820214/
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:ICVS - Artigos em Revistas Internacionais com Referee

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