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dc.contributor.authorLima, A. C.por
dc.contributor.authorPuga, Ana M.por
dc.contributor.authorMano, J. F.por
dc.contributor.authorConcheiro, Angelpor
dc.contributor.authorAlvarez-Lorenzo, Carmenpor
dc.identifier.citationLima A. C., Puga A. M., Mano J. F., Concheiro A., Alvarez-Lorenzo C. Free and copolymerized gamma-cyclodextrins regulate performance of dexamethasone-loaded dextran microspheres for bone regeneration, Journal of Materials Chemistry B, Vol. 2, Issue 30, pp. 4943-4956, doi:10.1039/C3TB21665A, 2014por
dc.description.abstractPolymeric particles acting as source of biological cues to promote tissue regeneration are currently an interesting topic in bone tissue engineering research. In this study, microspheres of dextran-methacrylated (dextran-MA) and γ-cyclodextrins (γ-CD) for the delivery of osteogenic agents were prepared by means of photopolymerization on biomimetic superhydrophobic surfaces. The effects of the incorporation of the γ-CD units as free entities or as structural monomers (acrylamidomethyl-γ-cyclodextrin, γ-CD-NMA) on dexamethasone loading and release performance were evaluated in detail in order to achieve osteogenic differentiation of human stem cells. The copolymerization of dextran-MA with γ-CD-NMA improved the loading capacity of the particles and also provided a sustained release of dexamethasone for several days. The biological studies revealed that such microspheres were cytocompatible and capable to induce the differentiation of human adipose-derived stem cells (hASCs) to osteoblasts, as determined from an increase of alkaline phosphatase (ALP) activity between days 3 and 7. Such results were also confirmed using ALP staining. Therefore, immobilization of γ-CDs onto dextran-MA network may be particularly useful for the development of cytocompatible implantable spherical biomaterials for bone tissue engineering purposes.por
dc.description.sponsorshipA.C. Lima acknowledges the Portuguese Foundation for Science and Technology (FCT) for the PhD grant SFRH/BD/71395/2010 and A.M. Puga thanks Ministerio de Economia y Competividad (MINECO) for an FPI fellowship (BES2009-024735). The research leading to these results has received funding from the European Union's Seventh Framework Programme (FP7/2007-2013) under grant agreement no. REGPOT-CT2012-316331-POLARIS, FEDER through the Competitive Factors Operation Program -COMPETE, National funds through FCT (PTDC/FIS/ 115048/2009 and PTDC/CTM-BIO/1814/2012), MINECO (SAF2011-22771) and Luso-Spanish Integrated Actions (ref. E122/12; PRI-AIBPT-2011-1211), and Operational Human Potential Program (POPH) developed under the scope of the National Strategic Reference Framework (QREN) from the European Social Fund (FSE). The authors would like to thank Prof. Asuncion Lage for the quantification of Irgacure 2959 released from the particles, and the Instituto de Ortopedia y Banco de Tejidos Musculoesqueleticos (Universidad de Santiago de Compostela, Spain) for help with cell cultures.por
dc.publisherRoyal Society of Chemistrypor
dc.subjectSuperhydrophobic surfacespor
dc.titleFree and copolymerized gamma-cyclodextrins regulate performance of dexamethasone-loaded dextran microspheres for bone regenerationpor
oaire.citationTitleJournal of Materials Chemistry Bpor
dc.subject.wosScience & Technologypor
sdum.journalJournal of Materials Chemistry Bpor
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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