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https://hdl.handle.net/1822/30306
Título: | Free and copolymerized gamma-cyclodextrins regulate performance of dexamethasone-loaded dextran microspheres for bone regeneration |
Autor(es): | Lima, A. C. Puga, Ana M. Mano, J. F. Concheiro, Angel Alvarez-Lorenzo, Carmen |
Palavras-chave: | Cyclodextrins Dextran Microspheres Superhydrophobic surfaces |
Data: | Mai-2014 |
Editora: | Royal Society of Chemistry |
Revista: | Journal of Materials Chemistry B |
Citação: | Lima A. C., Puga A. M., Mano J. F., Concheiro A., Alvarez-Lorenzo C. Free and copolymerized gamma-cyclodextrins regulate performance of dexamethasone-loaded dextran microspheres for bone regeneration, Journal of Materials Chemistry B, Vol. 2, Issue 30, pp. 4943-4956, doi:10.1039/C3TB21665A, 2014 |
Resumo(s): | Polymeric particles acting as source of biological cues to promote tissue regeneration are currently an interesting topic in bone tissue engineering research. In this study, microspheres of dextran-methacrylated (dextran-MA) and γ-cyclodextrins (γ-CD) for the delivery of osteogenic agents were prepared by means of photopolymerization on biomimetic superhydrophobic surfaces. The effects of the incorporation of the γ-CD units as free entities or as structural monomers (acrylamidomethyl-γ-cyclodextrin, γ-CD-NMA) on dexamethasone loading and release performance were evaluated in detail in order to achieve osteogenic differentiation of human stem cells. The copolymerization of dextran-MA with γ-CD-NMA improved the loading capacity of the particles and also provided a sustained release of dexamethasone for several days. The biological studies revealed that such microspheres were cytocompatible and capable to induce the differentiation of human adipose-derived stem cells (hASCs) to osteoblasts, as determined from an increase of alkaline phosphatase (ALP) activity between days 3 and 7. Such results were also confirmed using ALP staining. Therefore, immobilization of γ-CDs onto dextran-MA network may be particularly useful for the development of cytocompatible implantable spherical biomaterials for bone tissue engineering purposes. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/30306 |
DOI: | 10.1039/C3TB21665A |
ISSN: | 2050-750X |
Versão da editora: | http://pubs.rsc.org/en/content/articlelanding/2014/tb/c3tb21665a#!divAbstract |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18006-LimaAC_Journal of Materials Chemistry B_accepted manuscript.pdf Acesso restrito! | 1,54 MB | Adobe PDF | Ver/Abrir |