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TitleOptical projection tomography as a tool for 3D imaging of hydrogels
Author(s)Figueiras, E.
Soto, A. M.
Jesus, D.
Lehti, M.
Koivisto, J.
Parraga, J. E.
Silva-Correia, Joana
Oliveira, J. M.
Reis, R. L.
Kellomäki, M.
Hyttinen, J.
KeywordsGellan gum-based hydrogels
Optical projection tomography
Issue dateSep-2014
PublisherOptical Society of America
JournalBiomedical Optics Express
Abstract(s)An Optical Projection Tomography (OPT) system was developed and optimized to image 3D tissue engineered products based in hydrogels. We develop pre-reconstruction algorithms to get the best result from the reconstruction procedure, which include correction of the illumination and determination of sample center of rotation (CoR). Existing methods for CoR determination based on the detection of the maximum variance of reconstructed slices failed, so we develop a new CoR search method based in the detection of the variance sharpest local maximum. We show the capabilities of the system to give quantitative information of different types of hydrogels that may be useful in its characterization.
Publisher version
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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