Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/30169

TitleSurface modification of silica-based marine sponge bioceramics induce hydroxyapatite formation
Author(s)Barros, Alexandre A.
Aroso, Ivo Manuel Ascensão
Silva, Tiago H.
Mano, J. F.
Duarte, Ana Rita C.
Reis, R. L.
KeywordsHydroxyapatite
Marine sponge
Surface chemistry
Bioactivity
Biosilica
Scaffold
Tissue engineering
Issue dateAug-2014
PublisherAmerican Chemical Society
JournalCrystal Growth Design
CitationBarros A. A., Aroso I. M., Silva T. H., Mano J. F., Duarte A. R. C., Reis R. L. Surface modification of silica-based marine sponge bioceramics induce hydroxyapatite formation, Cryst. Growth Des, doi:10.1021/cg500654u, 2014
Abstract(s)Marine biomaterials are a new emerging area of research with significant applications. Recently, researchers are dedicating considerable attention to marine-sponge biomaterials for various applications. We have focused on the potential of biosilica from Petrosia ficidormis for novel biomedical/industrial applications. A bioceramic structure from this sponge was obtained after calcination at 750ºC for 6 hours in a furnace. The morphological characteristics of the 3D architecture were evaluated by scanning electron microscopy (SEM) and micro-computed tomography revealing a highly porous and interconnected structure. The skeleton of Petrosia ficidormis is a siliceous matrix composed of SiO2, which does not present inherent bioactivity. Induction of bioactivity was attained by subjecting the bioceramics structure to an alkaline treatment (KOH 2M) and acidic treatment (HCl 2M) for 1 and 3 hours. In vitro bioactivity of the bioceramics structure was evaluated in simulated body fluid (SBF), after 7 and 14 days. Observation of the structures by SEM, coupled with spectroscopic elemental analysis (EDS), has shown that the surface morphology presented a calcium-phosphate CaP coating, similar to hydroxyapatite (HA). The determination of the Ca/P ratio, together with the evaluation of the characteristic peaks of HA by infra-red spectroscopy and X-ray diffraction, have proven the existence of HA. In vitro biological performance of the structures was evaluated using an osteoblast cell line andthe acidic treatment has shown to be the most effective treatment. Cells were seeded on the bioceramics structures and their morphology, viability and growth was evaluated by SEM, MTS assay and DNA quantification, respectively, demonstrating that cells are able to grow and colonize the bioceramic structures.
Typearticle
URIhttp://hdl.handle.net/1822/30169
DOI10.1021/cg500654u
ISSN1528-7483
Publisher versionhttp://pubs.acs.org/doi/abs/10.1021/cg500654u
Peer-Reviewedyes
AccessopenAccess
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
18061-cg500654u.pdf2,53 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu Currículo DeGóis