Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/29616

TitlePhospholipase d2 ablation ameliorates Alzheimer's disease-linked synaptic dysfunction and cognitive deficits
Author(s)Oliveira, Tiago Gil
Chan, Robin B.
Tian, Huasong
Laredo, Mikael
Shui, Guanghou
Staniszewski, Agnieszka
Zhang, Hong
Wang, Lili
Kim, Tae-Wan
Duff, Karen E.
Wenk, Markus R.
Arancio, Ottavio
Di Paolo, Gilbert
Issue date8-Dec-2010
PublisherSociety for Neuroscience
JournalThe Journal of Neuroscience
Abstract(s)Growing evidence implicates aberrant lipid signaling in Alzheimer's disease (AD). While phospholipases A2 and C have been recently shown to mediate key actions of amyloid ß-peptide (Aß) through a dysregulation of arachidonic acid and phosphatidylinositol-4,5-bisphosphate metabolism, respectively, the role of phospholipase D (PLD) has so far remained elusive. PLD produces phosphatidic acid (PA), a bioactive lipid involved in multiple aspects of cell physiology, including signaling and membrane trafficking processes. Here we show that oligomeric Aß enhances PLD activity in cultured neurons and that this stimulatory effect does not occur upon ablation of PLD2 via gene targeting. Aß fails to suppress long-term potentiation in PLD2-deficient hippocampal slices, suggesting that PLD2 is required for the synaptotoxic action of this peptide. In vivo PLD activity, as assessed by detection of phosphatidylethanol levels using mass spectrometry (MS) following ethanol injection, is also increased in the brain of a transgenic mouse model of AD (SwAPP). Furthermore, Pld2 ablation rescues memory deficits and confers synaptic protection in SwAPP mice despite a significant Aß load. MS-based lipid analysis of Pld2 mutant brains in the presence or absence of the SwAPP transgene unmasks striking crosstalks between different PA species. This lipid analysis shows an exquisite acyl chain specificity and plasticity in the perturbation of PA metabolism. Collectively, our results point to specific molecular species of PA as key modulators of AD pathogenesis and identify PLD2 as a novel potential target for therapeutics.
TypeArticle
URIhttp://hdl.handle.net/1822/29616
DOI10.1523/JNEUROSCI.3317-10.2010
ISSN0270-6474
Publisher versionhttp://www.jneurosci.org/content/30/49/16419
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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