Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/29321

TítuloEndothelial cells enhance the in vivo bone-forming ability of osteogenic cell sheets
Autor(es)Pirraco, Rogério
Iwata, Takanori
Yoshida, T.
Marques, A.
Yamato, Masayuki
Reis, R. L.
Okano, T.
Palavras-chaveBone tissue engineering
Cell sheets
Vascularization
Endothelial cells
Osteoblasts
DataJun-2014
EditoraNature Publishing Group
RevistaLaboratory Investigation
CitaçãoPirraco R. P., Iwata T., Yoshida T., Marques A. P., Yamato M., Reis R. L., Okano T. Endothelial cells enhance the in vivo bone-forming ability of osteogenic cell sheets, Laboratory Investigation, Vol. 94, Issue 6, pp. 663-673, doi:10.1038/labinvest.2014.55, 2014
Resumo(s)Addressing the problem of vascularization is of vital importance when engineering three-dimensional (3D) tissues. Endothelial cells are increasingly used in tissue-engineered constructs to obtain prevascularization and to enhance in vivo neovascularization. Rat bone marrow stromal cells were cultured in thermoresponsive dishes under osteogenic conditions with human umbilical vein endothelial cells (HUVECs) to obtain homotypic or heterotypic cell sheets (CSs). Cells were retrieved as sheets from the dishes after incubation at 20 °C. Monoculture osteogenic CSs were stacked on top of homotypic or heterotypic CSs, and subcutaneously implanted in the dorsal flap of nude mice for 7 days. The implants showed mineralized tissue formation under both conditions. Transplanted osteogenic cells were found at the new tissue site, demonstrating CS bone-inductive effect. Perfused vessels, positive for human CD31, confirmed the contribution of HUVECs for the neovascularization of coculture CS constructs. Furthermore, calcium quantification and expression of osteocalcin and osterix genes were higher for the CS constructs, with HUVECs demonstrating the more robust osteogenic potential of these constructs. This work demonstrates the potential of using endothelial cells, combined with osteogenic CSs, to increase the in vivo vascularization of CS-based 3D constructs for bone tissue engineering purposes.
Tipoarticle
URIhttp://hdl.handle.net/1822/29321
DOI10.1038/labinvest.2014.55
ISSN0023-6837
Versão da editorahttp://www.nature.com/labinvest/journal/v94/n6/full/labinvest201455a.html
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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