Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/29290

TítuloPoly(hydroxybutyrate-co-hydroxyvalerate) bilayer skin tissue engineering constructs with improved epidermal rearrangement
Autor(es)Zonari, A. A. C.
Cerqueira, M. T.
Novikoff, S.
Goes, A. M.
Marques, A. P.
Correlo, V. M.
Reis, R. L.
Palavras-chaveBioengineering
Biomimetic
In vitro epidermal rearrangement
Polyesters
Wound healing
DataMar-2014
EditoraWiley Online Library
RevistaMacromolecular Bioscience
Resumo(s)Bilayer skin substitutes constitute an attractive strategy towards improved skinwound healing.Therefore, solvent casting and freeze-drying methodologies are used to produce polyhydroxybutyrate-co-hydroxyvalerate (PHBV) thin nanoporousmembranes and 3D porous scaffolds thatare combined in bilayer structures to recreate the epidermal and dermal layers, respectively. Thecombination of these methodologies allow attaining a bilayer structure with a high waterretention capability and adequate mechanical properties, susceptible to enzymes degradativeaction. Cultures established with human keratinocytes (hKC) and dermal fibroblasts(hDFb) confirm the suitability of the PHBV structures tosupport cell adhesion and proliferation. Nonetheless,when co-cultured under defined conditions, hKC are ableto grow and rearrange in a multilayer structure withproliferative cells in the basal layer, and cells expressing aterminal differentiation marker in the upper layer.Therefore, PHBV bilayer structures demonstrate propertiesthat favor skin cells performance, thus representing apromising strategy to improve wound healing.
Tipoarticle
Descrição"Article first published online: 4 MAR 2014 (epub ahead of print)"
URIhttp://hdl.handle.net/1822/29290
DOI10.1002/mabi.201400005
ISSN1616-5195
Versão da editorahttp://onlinelibrary.wiley.com/doi/10.1002/mabi.201400005/abstract
Arbitragem científicayes
AcessorestrictedAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
17988-Zonari et al, 2014 Macromolecular bioscience.pdf1,68 MBAdobe PDFVer/Abrir  Solicitar cópia ao autor!

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu Currículo DeGóis