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TitleAn improved method for high quality RNA isolation from needles of adult maritime pine trees
Other titlesRNA isolation from adult maritime pine needles
Author(s)Azevedo, Herlânder
Neto, T. Lino
Tavares, R. M.
KeywordscDNA library construction
Maritime pine needles
RNA isolation
Issue date2003
PublisherInternational Society for Plant Molecular Biology
JournalPlant molecular biology reporter
Citation"Plant molecular biology reporter". ISSN 0735-9640. 21:4 (2003) 333-338.
Abstract(s)Conventional RNA isolation methods optimised for pine seedlings have been shown to produce poor quality RNA when applied to needles of adult pine trees. We describe here a modified procedure to isolate high pure RNA from needles of thirty-year-old maritime pines, exhibiting high levels of phenolics, polysaccharides and RNases. Major changes were the inclusion of proteinase K in the extraction medium followed by incubation at 42°C. Integrity and purity were evaluated by denaturing gel electrophoresis and by spectrophotometry (A260/A230 and A260/A280). Total RNA could be successfully used for poly(A)+-RNA isolation and cDNA library construction.
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AccessOpen access
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