Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/28150

TitlePortuguese propolis disturbs glycolytic metabolism of human colorectal cancer in vitro
Author(s)Santos, Luísa Filipa Morais dos
Gonçalves, Vera Mónica Miranda
Baltazar, Fátima
Valença, Isabel
Ferreira, Ana Margarida
Aguiar, Cristina
KeywordsAntitumor activity
Colorectal cancer
Glycolytic metabolism
HCT-15 cells
Propolis
Issue date2013
PublisherSpringer
JournalBMC Complementary and Alternative Medicine
Abstract(s)Propolis is a resin collected by bees from plant buds and exudates, which is further processed through the activity of bee enzymes. Propolis has been shown to possess many biological and pharmacological properties, such as antimicrobial, antioxidant, immunostimulant and antitumor activities. Due to this bioactivity profile, this resin can become an alternative, economic and safe source of natural bioactive compounds.Antitumor action has been reported in vitro and in vivo for propolis extracts or its isolated compounds; however, Portuguese propolis has been little explored. The aim of this work was to evaluate the in vitro antitumor activity of Portuguese propolis on the human colon carcinoma cell line HCT-15, assessing the effect of different fractions (hexane, chloroform and ethanol residual) of a propolis ethanol extract on cell viability, proliferation, metabolism and death. METHODS: Propolis from Angra do Heroísmo (Azores) was extracted with ethanol and sequentially fractionated in solvents with increasing polarity, n-hexane and chloroform. To assess cell viability, cell proliferation and cell death, Sulforhodamine B, BrDU incorporation assay and Anexin V/Propidium iodide were used, respectively. Glycolytic metabolism was estimated using specific kits. RESULTS: All propolis samples exhibited a cytotoxic effect against tumor cells, in a dose- and time-dependent way. Chloroform fraction, the most enriched in phenolic compounds, appears to be the most active, both in terms of inhibition of viability and cell death. Data also show that this cytotoxicity involves disturbance in tumor cell glycolytic metabolism, seen by a decrease in glucose consumption and lactate production. CONCLUSION: Our results show that Portuguese propolis from Angra do Heroísmo (Azores) can be a potential therapeutic agent against human colorectal cancer.
TypeArticle
URIhttp://hdl.handle.net/1822/28150
DOI10.1186/1472-6882-13-184
ISSN1472-6882
Publisher versionhttp://www.biomedcentral.com/bmccomplementalternmed
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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