Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/27532

TitleLaccase coating of catheters with poly(catechin) for biofilm reduction
Author(s)Gonçalves, Idalina
Matamá, Maria Teresa
Paulo, Artur Cavaco
Silva, Carla Manuela Pereira Marinho da
KeywordsAntibiofilm
Adhesion
Catheters
Catechin
Laccase
Functionalization
Issue date2014
PublisherInforma Healthcare
JournalBiocatalysis and Biotransformation
Abstract(s)Urinary polyurethane (PU) and silicone (SI) catheters were coated with poly(catechin) to reduce bacterial adhesion. Laccase was used as a biocatalyst to oxidize the catechin monomer and produce the polymer. Optimization of the catheter surface functionalization followed two different approaches: with or without previous alkali treatment. The results indicated higher levels of polymer attachment for the alkali-treated catheters ( 18% for PU and  33% for SI catheters). The reduction of biofilm formation on the catheter surface was quantitatively evaluated under static adhesion conditions against Escherichia coli (96% reduction on PU) and Staphylococcus epidermidis (81% reduction on SI). The type of catheter material greatly influenced bacterial adhesion, though alkali treatment was consistently beneficial for poly(catechin) attachment and consequently for biofilm reduction.
TypeArticle
URIhttp://hdl.handle.net/1822/27532
DOI10.3109/10242422.2013.828711
ISSN1029-2446
e-ISSN1024-2422
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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