Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/27517

TitleActivated sludge characterization through microscopy : a review on quantitative image analysis and chemometric techniques
Author(s)Mesquita, D. P.
Amaral, A. L.
Ferreira, E. C.
KeywordsImage analysis
Activated sludge
Aggregates
Filaments
Morphology
Chemometric techniques
Issue date6-May-2013
PublisherElsevier
JournalAnalytica Chimica Acta
Abstract(s)In wastewater treatment processes, and particularly in activated sludge systems, efficiency is quite dependent on the operating conditions, and a number of problems may arise due to sludge structure and proliferation of specific microorganisms. In fact, bacterial communities and protozoa identification by microscopy inspection is already routinely employed in a considerable number of cases. Furthermore, quantitative image analysis techniques have been increasingly used throughout the years for the assessment of aggregates and filamentous bacteria properties. These procedures are able to provide an ever growing amount of data for wastewater treatment processes in which chemometric techniques can be a valuable tool. However, the determination of microbial communities’ properties remains a current challenge in spite of the great diversity of microscopy techniques applied. In this review, activated sludge characterization is discussed highlighting the aggregates structure and filamentous bacteria determination by image analysis on bright-field, phase-contrast, and fluorescence microscopy. An in-depth analysis is performed to summarize the many new findings that have been obtained, and future developments for these biological processes are further discussed.
TypeArticle
URIhttp://hdl.handle.net/1822/27517
DOI10.1016/j.aca.2013.09.016
ISSN0003-2670
e-ISSN1873-4324
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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